Isolation and Characterization of the Human Inter-α-Trypsin Inhibitor Family Heavy Chain-Related Protein (IHRP) Gene (ITIHL1)

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Author(s)

    • SAGUCHI Ken-ichi
    • Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University
    • TOBE Takashi
    • Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University
    • HASHIMOTO Ken
    • Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University
    • NAGASAKI Yuta
    • Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University
    • ODA Eiichi
    • Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University
    • NAKANO Yasuko
    • Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University
    • MIURA Nam-Ho
    • Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University
    • TOMITA Motowo
    • Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University

Abstract

Inter-α-trypsin inhibitor (ITI) family heavy chain-related protein (IHRP) is a novel human glycoprotein that shows significant homology in amino acid sequence to proteins of the ITI family heavy chains from human plasma. Three overlapping clones that encode the human inter-α-trypsin inhibitor family heavy chain-related protein (IHRP) gene (ITIHL1) were isolated and characterized. The IHRP gene spans 15 kb and is composed of 24 exons from 27 to 207 bp in size with consensus splice sites. The gene codes for the precursor of IHRP, which is similar to inter-α-trypsin inhibitor (ITI) family heavy chains. Two major transcription initiation sites were identified in the 5'-flanking region. They contain putative promoter elements, but no typical TATA box. Some exons of this gene showed significant similarities to those of the ITI-H1 gene in nucleotide length and in intron phasing. The tissue-specific transcription of this gene may be due to the presence of binding sites for the hepatocyte nuclear factors LF-A1, HNF-5, NF-IL6, and C/EBP. This gene was found to be localized very close to another unknown gene related to EST (GenBank accession #: R54643, R50663, R50563, H27139, and R54913).

Journal

  • The Journal of Biochemistry

    The Journal of Biochemistry 119(5), 898-905, 1996-05-01

    The Japanese Biochemical Society

References:  41

Cited by:  1

Codes

  • NII Article ID (NAID)
    10005185938
  • NII NACSIS-CAT ID (NCID)
    AA00694073
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    0021924X
  • Data Source
    CJP  CJPref  J-STAGE 
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