The 5'-Flanking Region of the Human Smooth Muscle Cell Calponin Gene Contains a cis-Acting Domain for Interaction with a Methylated DNA-Binding Transcription Repressor

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Author(s)

    • TAKAHASHI Katsuhito
    • Department of Medicine, Molecular Biology Unit, Osaka Medical Center for Cancer and Cardiovascular Diseases
    • OKADA Tsutomu
    • Pharmaceutical Research Laboratory, Kirin Brewery Co., Ltd.
    • OHGAMI Kinya
    • Pharmaceutical Research Laboratory, Kirin Brewery Co., Ltd.
    • MIKI Akiko
    • Department of Medicine, Molecular Biology Unit, Osaka Medical Center for Cancer and Cardiovascular Diseases
    • SHIBATA Nobuhiki
    • Department of Medicine, Molecular Biology Unit, Osaka Medical Center for Cancer and Cardiovascular Diseases

Abstract

The human smooth muscle cell (SMC) calponin gene, which is composed of seven exons and six introns, spanning an approximately 11.2 kilobase (kb) genomic DNA, has been isolated and characterized by sequence analysis. As determined by primer extension mapping and rapid amplification of mRNA transcripts, a major transcription start site of the calponin gene is located at 101 base-pairs (bp) upstream of the ATG start codon. A striking feature of the 5'-flanking, 5'-untranslated, and amino-terminal protein coding regions is the presence of an <i>Alu</i> repetitive sequence and a stretch of DNA sequence identified using a methyl CpG-binding protein affinity column [Cross, S. H., Charlton, J. A., Nan, X., and Bird, A. P. (1994) <i>Nature Genet</i>. 6, 236-244]. The results of this study provide a new insight into the molecular mechanism underlying regulation of SMC-lineage specific gene expression.

Journal

  • The Journal of Biochemistry

    The Journal of Biochemistry 120(1), 18-21, 1996-07-01

    The Japanese Biochemical Society

References:  39

Cited by:  3

Codes

  • NII Article ID (NAID)
    10005187510
  • NII NACSIS-CAT ID (NCID)
    AA00694073
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    0021924X
  • Data Source
    CJP  CJPref  J-STAGE 
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