The rpoD1 Gene Product Is a Principal Sigma Factor of RNA Polymerase in Microcystis aeruginosa K-81

• ASAYAMA Munehiko

  Division of Biotechnology, School of Agriculture, Ibaraki University

• SUZUKI Hidechika

  Division of Biotechnology, School of Agriculture, Ibaraki University

• SATO Akio

  Division of Biotechnology, School of Agriculture, Ibaraki University

• AIDA Tokujiro

  Division of Biotechnology, School of Agriculture, Ibaraki University

• TANAKA Kan

  Institute of Molecular and Cellular Biosciences, The University of Tokyo

• TAKAHASHI Hideo

  Institute of Molecular and Cellular Biosciences, The University of Tokyo

• SHIRAI Makoto

  Division of Biotechnology, School of Agriculture, Ibaraki University

抄録

We performed molecular characterization of the RpoDl protein encoded by the rpoD1 gene isolated from a cyanobacterium, Microcystis aeruginosa K-81. The deduced amino acid sequence (416 aa, 48, 871 Da) of RpoDl exhibited extensive similarity to those of proteins of the eubacterial RpoD family (Escherichia coli σ⁷⁰ homologs). We overproduced and purified RpoDl (54 kDa) from E. coli. Biological and biochemical analyses suggested that RpoD1 has a function homologous to that of E. coli σ⁷⁰ as follows: (i) the RpoD1 protein complemented an rpoD mutant of E. coli strain YN543 (rpoD285) and (ii) the heterologous RNA polymerase holoenzyme reconstituted from the E. coli core enzyme and recombinant RpoD1 was specifically transcribed from E. coli promoters. Furthermore, Western blot analysis with antiserum against Synechococcus sp. strain PCC 7942 RpoD1 (a principal sigma factor of the σ⁷⁰ type) indicated that M. aeruginosa K-81 RpoDl (σ^A1) is the principal σ factor, which is a major component of the σ subunit on exponential cell growth.

収録刊行物

• The journal of biochemistry

  The journal of biochemistry 120 (4), 752-758, 1996-10-01

  社団法人 日本生化学会

キーワード

• cyanobacteria
• RNA polymerase
• sigma factor
• transcription
• Western blot

詳細情報

• CRID: 1571980074277323264
• NII論文ID: 10005190321
• NII書誌ID: AA00694073
• ISSN: 0021924X
• Web Site: http://chemport.cas.org/cgi-bin/sdcgi?APP=ftslink&action=reflink&origin=jstage2&version=1.0&coi=1:CAS:528:DyaK28Xms1Wntbc%3D&md5=bb53bbd24f9fac88ad2b178978266be9
• 本文言語コード: en
• データソース種別:
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