RAPD法による常緑性ツツジの品種同定

DOI 日本農学文献記事索引 Web Site Web Site 被引用文献14件 参考文献19件

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タイトル別名
  • Cultivar Identification of Evergreen Azalea with RAPD Method.
  • RAPD法による常緑性ツツジの品種同定〔英文〕
  • RAPDホウ ニヨル ジョウリョクセイ ツツジ ノ ヒンシュ ドウテイ エイブ

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To use RAPD method for the identification of evergreen azalea cultivars, the optimum method for DNA extraction in Rhododendron spp. was investigated. Of almost all Rhododendron species native to Japan and some cultivar groups, neither SDS nor CTAB method was sufficient to isolate clean DNA from leaves. But, by using the modified CTAB method after Greenwood et al. (1989), we obtained a good yield of high quality DNA from the materials used in this experiment.<BR>Using the optimum extraction method mentioned above and RAPD analysis, differences among azalea cultivars could be distinguished. Sixteen out of 100 primers tested produced obvious, definitive polymorphic DNA bands. Cultivars in the Edo-Kirishima group (9 cultivars) or Kurume azalea group (14 cultivars) were clearly recognized from one another by using primer OPK-19 or OPK-20. In the Satsuki group, these two primers could be used to discern 'Kouzan' from its bud sport cultivars.<BR>The results of this study strongly suggest that RAPD method is a very useful tool for cultivar identification in evergreen azalea.

収録刊行物

  • 園芸学会雑誌

    園芸学会雑誌 64 (3), 611-616, 1995

    一般社団法人　園芸学会

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