Probing the Secondary Structure of MCS4 RNA of Mycoplasma capricolun

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Author(s)

Abstract

MCS4 RNA is one of the small stable RNAs found in <i>Mycoplasma capricolum</i>. Its function is unknown. The conformation of MCS4 RNA (125 nucleotides in length) in solution was investigated using chemical and enzymatic probes. Single and double-stranded regions were estimated by means of diethyl pyrocarbonate (DEPC) and by dimethyl sulfate (DMS) modifications. Ribonuclease V<sub>1</sub> was also used to identify paired or stacked nucleotides. Based on these data, a secondary structure model for MCS4 RNA containing four stemstructures was constructed.

Journal

  • The Journal of Biochemistry

    The Journal of Biochemistry 122(6), 1202-1207, 1997-12-01

    The Japanese Biochemical Society

References:  23

Cited by:  1

Codes

  • NII Article ID (NAID)
    10005460900
  • NII NACSIS-CAT ID (NCID)
    AA00694073
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    0021924X
  • NDL Article ID
    4357318
  • NDL Source Classification
    ZR2(科学技術--生物学--生化学)
  • NDL Call No.
    Z53-B472
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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