Large-scale Formation and Continuous Suspension Culture of Porcine Hepatocyte Spheroids.
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- SAKAI Y.
- Institute of Industrial Science, University of Tokyo
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- NARUSE K.
- 1st Dept. of Surg., Fac. of Med., Univ. of Tokyo
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- NAGASHIMA I.
- 1st Dept. of Surg., Fac. of Med., Univ. of Tokyo
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- MUTO T.
- 1st Dept. of Surg., Fac. of Med., Univ. of Tokyo
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- SUZUKI M.
- Institute of Industrial Science, University of Tokyo
Bibliographic Information
- Other Title
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- ブタ肝細胞スフェロイドの大量形成とその連続浮遊培養
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Abstract
Approximately a 1/4 cell number of porcine hepatocytes needed for a bioartificial liver for a pig (10-15kg) with liver failure was successfully re-organized to spheroids within 24 hours by 1-L scale suspension culture vessel fitted oxigen supply apparatus. Although the cell number was gradually decreased in subsequent continuous suspension culture of the spheroids during 10 days, the functional expressions per cell of the spheroids were 3-5 times higher as compared to those of monolayers. Collagen gel entrapment of the spheroids lowered the functional expressions. No deteriolation was observed in the function of the spheroids in suspension culture even in direct contact with 100% porcine or human plasma.
Journal
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- Jinko Zoki
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Jinko Zoki 24 (1), 18-23, 1995
JAPANESE SOCIETY FOR ARTIFICIAL ORGANS
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Details 詳細情報について
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- CRID
- 1390001204954122880
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- NII Article ID
- 10005624070
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- NII Book ID
- AN00120167
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- ISSN
- 18836097
- 03000818
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- Text Lang
- ja
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed