Evaluation of Blood Compatibility of 6 Different Polyurethanes Using an Epifluorescent Video Microscopy.

• KURODA S.

  Dept. of Chem. Eng., Waseda Univ.

• NOJIRI C.

  Inst. of Biomed. Sci., Terumo Corp.

• KIDO T.

  Inst. of Biomed. Sci., Terumo Corp.

• SENSHU K.

  Inst. of Biomed. Sci., Terumo Corp.

• NAGAI H.

  Inst. of Biomed. Sci., Terumo Corp.

• SAKAI K.

  Dept. of Chem. Eng., Waseda Univ.

Bibliographic Information

Other Title

• Ｅｐｉｆｌｕｏｒｅｓｃｅｎｔ　Ｖｉｄｅｏ　Ｍｉｃｒｏｓｃｏｐｙ（ＥＶＭ）を用いた６種類の異なるセグメント化ポリウレタンの抗血栓性評価

Abstract

We have evaluated blood compatibility of six different segmented polyurethanes (PUs) including five polyether-PUs and one polyurethane-urea (PUU) having PTMG as a soft segment, using our epifluorescent video microscopy (EVM) combined with a parallel plate flow cell. HEMA/Styrene block copolymer (HEMA/St), which we have already proven to be an excellent nonthrombogen is polymer over existing PUs, was used as a control. EVM system measured the amount of adhered platelet on the surfaces under the flow of human whole blood containing mepacrine labelled platelets perfused at a wall shear rate of 200/sec every one minute intervals for 20min. Platelet activation (β-TG) and complement activation (C3a) were also measured. PUU showed significantly higher platelet adhesional behavior than the other PUs. On the other hand, PU-PTMG (MW 1500) showed lowest platelet adhesional behavior among six PUs, which was comparable to HEMA/St. β-TG level of each polymer was somewhat related to platelet adhesional tendency. Complement activation was inversely correlated with the results of platelet adhesion and activation potentials except HEMA/St, which showed least platelet activation and complement activation. From the chemical compositions, we could divide these PUs into three categories: 1) PUU, 2) PU with lower MW of PTMG, 3) PU with higher MW of PTMG. From our experimental results, we could confirm that these PUs were divided into three categories above mentioned, i. e., 1) PU with higher MW of PTMG showed lesser platelet adhesion and higher complement activation than PU with lower MW of PTMG, 2) PUU showed the highest platelet adhesion. Thus, blood compatibility of PUs were much influenced by their chemical compositions.

Journal

• Jinko Zoki

  Jinko Zoki 24 (1), 59-64, 1995

  JAPANESE SOCIETY FOR ARTIFICIAL ORGANS

Keywords

• epifluorescent video microscopy (EVM)
• segmented polyurethane (PU)
• platelet
• flow chamber
• mepacrine

Details

• CRID: 1390001204957062912
• NII Article ID: 10005624189
• NII Book ID: AN00120167
• DOI: 10.11392/jsao1972.24.59
• ISSN: 18836097
• ISSN: 03000818
• Web Site: https://search.jamas.or.jp/link/ui/1995164376
• Text Lang: ja
• Data Source:
  • JaLC
  • CiNii Articles
• Abstract License Flag: Disallowed