A Healthy Carrier of Verotoxin-Producing <I>Escherichia coli</I>, Who Was Detected by Periodic Feces Examinations

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  • MURATA Yutaka
    Shionogi Biomedical Laboratories, Shionogi Pharmaceutical Co., Ltd.
  • KUSUNOKI Shinji
    Shionogi Biomedical Laboratories, Shionogi Pharmaceutical Co., Ltd.
  • MINAMIDE Wakio
    Shionogi Biomedical Laboratories, Shionogi Pharmaceutical Co., Ltd.
  • IGIMI Hirotune
    Shionogi Biomedical Laboratories, Shionogi Pharmaceutical Co., Ltd.
  • UCHIDA Kiyohisa
    Shionogi Biomedical Laboratories, Shionogi Pharmaceutical Co., Ltd.
  • TAKEDA Tae
    Department of Infectious Diseases Research, National Children's Research Center

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Other Title
  • 定期検便で検出されたVero毒素産生性大腸菌 (O128: H9) の健康保菌者
  • テイキ ケンベン デ ケンシュツサレタ Vero ドクソ サンセイセイ ダイチ
  • A Healthy Carrier of Verotoxin-Producing Escherichia coli, Who Was Detected by Periodic Feces Examinations

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Abstract

Verotoxin-producing Escherichia coli (VTEC) was rapidly detected by the PCR method in one of the 9 feces samples. They were collected from the people who had been cooking meals for patients on a periodic feces examination on November 25, 1992. It was confirmed by PCR that the isolate had both VT1 and VT2vh toxic genes and showed cytotoxity on Verocells. In this case, the isolate was highly susceptible to common antibiotic agents, it was removed by the administration of tosfulaxacin. Some isolates which had the same properties as those of the strain described in “VITEK GNI card” and the antimicrobial susceptibility and VT toxin types, were detected in 2 of the 3 members of her family, they were healthy carriers and did not have any subjective symptoms such as diarrhea or abdominal pain.<BR>In order to detect Verotoxic genes, the sample of feces preincubated for 3 h in trypticase broth was subjected to PCR.<BR>We recommend that this method is much more useful because of rapid detection and identification of VTEC compared with the classical culture method.

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 67 (12), 1211-1218, 1993

    The Japanese Association for Infectious Diseases

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