A Survey of Genes Expressed in Mouse Embryonal Carcinoma F9 Cells : Characterization of Expressed Sequence Tags Matching No Known Genes

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Author(s)

    • Nomura Midori NOMURA Midori
    • Department of Medical Genetics, Division of Molecular Biomedicine, Research Institute for Microbial Diseases, Osaka University
    • TAKIHARA Yoshihiro
    • Department of Medical Genetics, Division of Molecular Biomedicine, Research Institute for Microbial Diseases, Osaka University
    • SHIMADA Kazunori
    • Department of Medical Genetics, Division of Molecular Biomedicine, Research Institute for Microbial Diseases, Osaka University

Abstract

We prepared 2, 132 expressed sequence tags (ESTs) from undifferentiated mouse embryonal carcinoma F9 cells and found that 1, 416 match known gene and/or protein sequences [Nishiguchi <i>et al</i>. (1996) <i>J. Biochem</i>. 119, 749-767]. To obtain information on the functions of the remaining 716 unidentified ESTs and to develop a system for characterizing ESTs matching no known genes, we analyzed their sequences by (i) repeated database searches, using the BLASTN, BLASTX, TBLASTX, and FASTA programs, (ii) using computer programs developed or modified for this work, such as the WFASTA, ORFTRNS, and MFASTA programs, together with the DBPROSITE and GRAIL programs, and (iii) examining the expression patterns of the corresponding mRNAs in F9 cells and several organs of adult mice, using the digoxigenin-labeled dot-blot method. We found that 216 of the 716 ESTs match known gene and/or protein sequences, and 307 show significant similarities to these sequences, with a Poisson <i>p</i>-value <0.01. The strategy and usefulness of such analysis for characterizing unidentified ESTs are discussed.

Journal

  • The Journal of Biochemistry

    The Journal of Biochemistry 122(1), 129-147, 1997-07-01

    The Japanese Biochemical Society

References:  16

Codes

  • NII Article ID (NAID)
    10005836471
  • NII NACSIS-CAT ID (NCID)
    AA00694073
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    0021924X
  • NDL Article ID
    4251882
  • NDL Source Classification
    ZR2(科学技術--生物学--生化学)
  • NDL Call No.
    Z53-B472
  • Data Source
    CJP  NDL  J-STAGE 
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