Connective tissue growth factor induces the proliferation, migration, and tube formation of vascular endothelial cells

• Shimo Tsuyoshi

  Department of Biochemistry and Molecular Dentistry, Okayama University Dental School Department of Oral and Maxillofacial Surgery II, Okayama University Dental School

• Nakanishi Tohru

  Department of Biochemistry and Molecular Dentistry, Okayama University Dental School Biodental Research Center Okayama University Dental School

• Nishida Takashi

  Department of Biochemistry and Molecular Dentistry, Okayama University Dental School Biodental Research Center Okayama University Dental School Department of Periodontology and Endodontology II, Okayama University Dental School

• Asano Masahiro

  Department of Biochemistry and Molecular Dentistry, Okayama University Dental School Department of Periodontology and Endodontology II, Okayama University Dental School

• Kanyama Manabu

  Department of Fixed Prosthodontics, Okayama University Dental School

• Kuboki Takuo

  Department of Fixed Prosthodontics, Okayama University Dental School

• Tamatani Takuya

  Pharmaceutical Frontier Research Laboratories, Japan Tobacco Inc.

• Tezuka Katsunari

  Pharmaceutical Frontier Research Laboratories, Japan Tobacco Inc.

• Takemura Motohide

  Department of Oral Anatomy, Osaka University Faculty of Dentistry

• Matsumura Tomohiro

  Department of Oral and Maxillofacial Surgery II, Okayama University Dental School

• Takigawa Masaharu

  Department of Biochemistry and Molecular Dentistry, Okayama University Dental School Biodental Research Center Okayama University Dental School

書誌事項

タイトル別名

• Connective Tissue Growth Factor Induces the Proliferation, Migration, and Tube Formation of Vascular Endothelial Cells In Vitro, and Angiogenesis In Vivo.

抄録

Connective tissue growth factor (CTGF) is a novel cysteine-rich, secreted protein. Recently, we found that inhibition of the endogenous expression of CTGF by its antisense oligonucleotide and antisense RNA suppresses the proliferation and migration of vascular endothelial cells. In the present study, the following observations demonstrated the angiogenic function of CTGF in vitro and in vivo: (i) purified recombinant CTGF (rCTGF) promoted the adhesion, proliferation and migration of vascular endothelial cells in a dose-dependent manner under serum-free conditions, and these effects were inhibited by anti-CTGF antibodies; (ii) rCTGF markedly induced the tube formation of vascular endothelial cells, and this effect was stronger than that of basic fibroblast growth factor or vascular endothelial growth factor; (iii) application of rCTGF to the chicken chorioallantoic membrane resulted in a gross angiogenic response, and this effect was also inhibited by anti-CTGF antibodies. (iv) rCTGF injected with collagen gel into the backs of mice induced strong angiogenesis in vivo. These findings indicate that CTGF is a novel, potent angiogenesis factor which functions in multi-stages in this process.

収録刊行物

• The Journal of Biochemistry

  The Journal of Biochemistry 126 (1), 137-145, 1999

  社団法人 日本生化学会

キーワード

• angiogenesis
• connective tissue growth factor (CTGF)
• endothelial cells
• hypertrophic chondrocytes
• neovascularization

詳細情報

• CRID: 1390282679908849024
• NII論文ID: 130003533672
• NII論文ID: 10005856662
• NII書誌ID: AA00694073
• DOI: 10.1093/oxfordjournals.jbchem.a022414
• COI: 1:CAS:528:DyaK1MXmtlagtLo%3D
• ISSN: 17562651
• ISSN: 0021924X
• ISSN: http://id.crossref.org/issn/0021924X
• NDL書誌ID: 4785588
• PubMed: 10393331
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4785588
• Web Site: http://academic.oup.com/jb/article-pdf/126/1/137/2498473/126-1-137.pdf
• Web Site: https://search.jamas.or.jp/link/ui/1999256597
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可