Enhanced Ecpression of Fc Receptors on Neutrophils from Calves with Leukocyte Adhesion Deficiency

• NAGAHATA Hajime

  Department of Animal Health, School of Veterinary Medicine, Rakuno Gakuen University

• HIGUCHI Hidetoshi

  Department of Animal Health, School of Veterinary Medicine, Rakuno Gakuen University

• NOCHI Hiromi

  Department of Microbiology, Faculty of Pharmaceutical Science, Health Science University of Hokkaido

• TAMOTO Koichi

  Department of Microbiology, Faculty of Pharmaceutical Science, Health Science University of Hokkaido

• NODA Hiroshi

  Department of Animal Health, School of Veterinary Medicine, Rakuno Gakuen University

• KOCIBA Gary J

  Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University

抄録

The expression of Fc receptors for immunoglobulin G(IgG) and concanavalin A (con A)-binding receptors, luminol-dependent chemiluminescent (LDCL) responses, and the effect of anti-bovine IgG on LDCL responses were evaluated in neutrophils from Holstein calves with leukocyte adhesion deficiency (BLAD). Neutrophils from affected calves showed a 2.1- to 2.5-fold increase in Fc receptor expression compared with those of control calves by flow cytometric analysis. Con A-binding activities of neutrophils from affected calves were similar to those of control calves. Neutrophils from a calf with BLAD, when stimulated with zymosan opsonized with bovine serum (OPZ), heat-aggregated bovine IgG (Agg-bovine IgG), sheep red blood cells (SRBC) sensitized with anti-SRBC antibody (SRBC-anti-SRBC Ab), or con A had LDCL responses of 36 (P<0.05), 77, 126 and 119% of peak LDCL values of controls, respectively. The NBT reducing value of neutrophils from a calf with BLAD when stimulated with Agg-bovine IgG after pretreatment with anti-bovine IgG was 116.5% of the values of neutrophils from control calves, but the difference was not significant. The LDCL responses of neutrophils from a control calf and a calf with BLAD stimulated with OPZ were inhibited markedly by pre-incubation with anti-bovine IgG antiserum at concentrations ranging from 1.25 to 20 or 40μg/ml. Although an increase in Fc receptor expression on neutrophils from calves with BLAD was observed, the LDCL responses stimulated with SRBC-anti-SRBC Ab and NBT-reducing activity stimulated with Agg-bovine IgG after pretreatment with anti-bovine IgG did not correlate significantly with the increased Fc receptor expression. These results support that neutrophil functions mediated by the Fc receptors are associated synergistically with the presence of the complement receptor type 3 (CR3)(CD11b/CD18).

収録刊行物

• Microbiology and immunology

  Microbiology and immunology 39 (9), 703-308, 1995-09-20

  財団法人 学会誌刊行センター

キーワード

• Bovine LAD
• Neurtophils
• Fc receptor
• CR3

詳細情報

• CRID: 1571698599323270656
• NII論文ID: 10005869998
• NII書誌ID: AA00738350
• ISSN: 03855600
• Web Site: http://chemport.cas.org/cgi-bin/sdcgi?APP=ftslink&action=reflink&origin=jstage2&version=1.0&coi=1:CAS:528:DyaK2MXptVahu74%3D&md5=5ccd6047f3c44989c2bc83be7a13e6f2
• 本文言語コード: en
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