胚珠培養によるリーガルユリとヒメサユリの種間雑種の作出

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タイトル別名
  • Production of Interspecific Hybrids between Lilium regale and L. rubellum via Ovule Culture.
  • Production of interspecific hybrids bet

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Lilium regale and L. rubellum were crossed reciprocally to introduce economically and horticulturally desirable traits of L. regale, such as vigorous growth and disease resistance, into L. rubellum.<BR>1. In L. regale ×L. rubellum, seeds containing an embryo were obtained with a low frequency (3.3 %) by stigmatic pollination at anthesis, but they did not germinate in soil or in vitro. Seedlings were successfully obtained with frequencies of 5.3 to 6.7 % by applying ovule culture 30 to 60 days after pollination.<BR>2. In L. rubellum ×L. regale, the growth of pollen tubes was inhibited in the style after stigmatic pollination at anthesis; hence, no ovule with an embryo was obtained. Growth of pollen tubes was promoted by stigmatic pollination when the pollination was done 2 to 5 days after anthesis. Embryos definitely formed if stigmatic pollination was carried out 5 days after anthesis, but they could not be rescued even when ovule culture was utilized. Cut-style pollination had no effect on production of ovules with an embryo.<BR>3. Hybridity of seedlings resulted from L. regale × L. rubellum was confirmed by nuclear rDNA analysis. All of the hybrid plants analyzed were diploid with 2n = 24 chromosomes and showed pollen fertilities of below 3 %. Flower color of the hybrids was light pink. A single hybrid clone produced a double flower.

収録刊行物

  • 園芸学会雑誌

    園芸学会雑誌 64 (4), 919-925, 1996

    一般社団法人　園芸学会

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