スイートオレンジ未熟種子由来のcDNAライブラリーの部分塩基配列の解析

• 久田 素

  明治大学農学部

• 森口 卓哉

  果樹試験場カンキツ部興津

• 日高 哲志

  果樹試験場カンキツ部興津 国際農林水産業研究センター沖縄支所

• Koltunow Anna M.

  CSIRO Divisiton of Horticulture

• 秋濱 友也

  明治大学農学部

• 大村 三男

  果樹試験場カンキツ部興津

書誌事項

タイトル別名

• Random Sequencing of Sweet Orange (<I>Citrus sinensis</I> Osbeck) cDNA Library Derived from Young Seeds
• Random sequencing of sweet orange(Citrus sinensis Osbeck)cDNA library derived from young seeds
• Random sequencing of sweet orange Citru

抄録

Sequencing of randomly selected cDNA clones was carried out to survey the repertoire of genes expressed in immature seeds of 'Valencia' orange (Citrus sinensis Osbeck) and to produce restriciton fragment length polymorphism (RFLP) markers on the genome mapping program in Citrus. A cDNA library was made by using mRNA prepared from immature seeds of 'Valencia' orange whose developing embryos were at an early globular stage. A total of 192 individual cDNA clones were partially sequenced and compared with the GenBank database. The cDNA clones, which presented significant homology to previously characterized plant, eukaryote, and all other organism genes (optimized (OPT) score >200) in data source formed 24.5% (47 clones), 12.0% (23 clones) and 8.8% (17 clones) of total cDNA clones analyzed in this experiment. The seed cDNA library showed low level of redundancy of cDNA clones. Some clones such as metallothionein, however, were revealed several times, suggesting possible isoforms. Although putative genes, which are thought to play a role on embryogenesis, were not screened in this experiment, a wide range of genes involved in functions such as metabolism, translation, signal transduction, and stress responses were obtained. The randomly sequenced cDNA clones described in this study provide, for the first time, an indication of the types of genes expressed at an early stage of seed development in Citrus. Furthermore, this method made possible the selection of many gene species which could be used as RFLP probes.

収録刊行物

• 園芸学会雑誌

  園芸学会雑誌 65 (3), 487-495, 1996

  一般社団法人　園芸学会

キーワード

• Horticulture
• General Earth and Planetary Sciences
• General Engineering
• General Environmental Science

詳細情報

• CRID: 1390282681280651008
• NII論文ID: 10006100459
• NII書誌ID: AN00025257
• DOI: 10.2503/jjshs.65.487
• COI: 1:CAS:528:DyaK28XnsFylu7Y%3D
• ISSN: 1880358X
• ISSN: 00137626
• NDL書誌ID: 4096882
• Web Site: https://agriknowledge.affrc.go.jp/RN/2010541960
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4096882
• Web Site: http://www.jstage.jst.go.jp/article/jjshs1925/65/3/65_3_487/_pdf
• 本文言語コード: en
• データソース種別:
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  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可