ＰＣＲ法によるアカグサレ病菌卵胞子の滅菌泥からの検出

• 横尾 一成

  佐賀県有明水産振興センター

• 川村 嘉応

  佐賀県有明水産振興センター

• 川原 逸朗

  佐賀県有明水産振興センター

• 東條 元昭

  大阪府立大学大学院農学生命科学研究科

• 水上 譲

  水産大学校

書誌事項

タイトル別名

• Detection of Oospore of Pythium porphyrae from Sterile Sediments by Using PCR.

抄録

Oospores of Pythium porphyrae in sterile sediments were detected by using PCR technique. In the PCR reactions, the 10-mer arbitrary primer (OPB-07) and one pair of 24-mer primers were used. The latter primers were designed from the DNA sequence data of P. porphyrae genomic DNA, and desingnated as AP-I and II. DNA of oospores in sterile sediments were amplified by the OPB-07 primer in the 1st PCR. However, the quantity of amplified DNA was found to be not significant. Detectable amounts and the products of expected sizes for P. porphyrae were amplified in the 2nd PCR (Nested PCR) using AP-I and II primers. These results indicate that the PCR primers developed in this study are useful for the detection of P. porphyrae oospores in sediments.

収録刊行物

• 水産増殖

  水産増殖 48 (4), 679-680, 2000

  日本水産増殖学会

キーワード

• <I>Pythium porphyrae</I>
• Oospore
• Nested PCR

詳細情報

• CRID: 1390282679692496640
• NII論文ID: 10006325492
• NII書誌ID: AN00124667
• DOI: 10.11233/aquaculturesci1953.48.679
• ISSN: 21850194
• ISSN: 03714217
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可