A Simple Identification Method for Spore-forming Bacteria Showing High Resistance against .GAMMA.-Rays.

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  • γ線抵抗性の強い芽胞形成菌の簡易同定法
  • ガンマセン テイコウセイ ノ ツヨイ ガホウ ケイセイキン ノ カンイ ドウテ

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Abstract

A simple identification method was developed for spore-forming bacteria which are highly resistant against γ-rays.<BR>Among 23 species of Bacillus studied, the spores of Bacillus megaterium, B. cereus, B. thuringiensis, B. pumilus and B. aneurinolyticus showed high resistance against γ-rays as compared with other spores of Bacillus species.<BR>Combination of the seven kinds of biochemical tests, namely, the citrate utilization test, nitrate reduction test, starch hydrolysis test, Voges-Proskauer reaction test, gelatin hydrolysis test, mannitol utilization test and xylose utilization test showed a characteristic pattern for each species of Bacillus. The combination pattern of each the above tests with a few supplementary test, if necessary, was useful to identify Bacillus species showing high radiation resistance against γ-rays.<BR>The method is specific for B. megaterium, B. thuringiensis and B. pumilus, and highly selective for B. aneurinolyticus and B. cereus.

Journal

  • RADIOISOTOPES

    RADIOISOTOPES 42 (11), 614-623, 1993

    Japan Radioisotope Association

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