Ser787 in the Proline-Rich Region of Human MAP4 is a Critical Phosphorylation Site that Reduces its Activity to Promote Tublin Polymerization

• Kitazawa Hidefumi

  Department of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University, 1-1 Minami-ohsawa, Hachiohji, Tokyo 192-0397, Laboratory of Cell and Developmental Biology, Faculty of Biosciences, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8501,

• Iida Junko

  Department of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University, 1-1 Minami-ohsawa, Hachiohji, Tokyo 192-0397,

• Uchida Atsuko

  Department of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University, 1-1 Minami-ohsawa, Hachiohji, Tokyo 192-0397,

• Haino-Fukushima Kazu

  Department of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University, 1-1 Minami-ohsawa, Hachiohji, Tokyo 192-0397,

• J. Itoh Tomohiko

  Division of Biological Sciences, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8602,

• Hotani Hirokazu

  Division of Biological Sciences, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8602,

• Ookata Kayoko

  Laboratory of Cell and Developmental Biology, Faculty of Biosciences, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8501,

• Murofushi Hiromu

  Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan, and

• Chloe Bulinski Jeannette

  Department of Anatomy and Cell Biology, College of Physicians and Surgeons of Columbia University, New York, New York 10032

• Kishimoto Takeo

  Laboratory of Cell and Developmental Biology, Faculty of Biosciences, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8501,

• Hisanaga Shin-ichi

  Department of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University, 1-1 Minami-ohsawa, Hachiohji, Tokyo 192-0397,

書誌事項

タイトル別名

• Ser787 in the Proline-Rich Region of Human MAP4 is a Critical Phosphorylation Site that Reduces its Activity to Promote Tubulin Polymerization.

抄録

p34^cdc2 kinase-phosphorylation sites in the microtubule (MT)-binding region of MAP4 were determined by peptide sequence of phosphorylated MTB3, a fragment containing the carboxy-terminal half of human MAP4. In addition to two phosphopeptides containing Ser696 and Ser787 which were previously indicated to be in vivo phosphorylation sites, two novel phosphopeptides, containing Thr892 or Thr901 and Thr917 as possible phosphorylation sites, were isolated, though only in in vitro phosphorylation. The role of phosphorylation at Ser696 and Ser787, which were differently phosphorylated during the cell cycle (Ookata et al., (1997). Biochemistry, 36: 15873-15883), was investigated in MT-polymerization, using MAP4 Ser to Glu mutants, which mimic phosphorylation at each site. Mutation of Ser787 to Glu strikingly reduced the MAP4's MT-polymerization activity, while Glu-mutation at Ser696 did not. These results suggest that Ser787 could be the critical phosphorylation site causing MTs to be dynamic at mitosis.

収録刊行物

• Cell Structure and Function

  Cell Structure and Function 25 (1), 33-39, 2000

  日本細胞生物学会

キーワード

• microtubule
• MAPs
• MAP4
• cdc2 kinase
• phosphorylation
• mitosis

詳細情報

• CRID: 1390001204694418944
• NII論文ID: 10006678784
• NII書誌ID: AA0060007X
• DOI: 10.1247/csf.25.33
• COI: 1:CAS:528:DC%2BD3cXjtVyhu74%3D
• ISSN: 13473700
• ISSN: 03867196
• NDL書誌ID: 5353156
• PubMed: 10791892
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I5353156
• Web Site: http://www.jstage.jst.go.jp/article/csf/25/1/25_1_33/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可