Construction of a BAC library derived from the inbred Hd-rR strain of the teleost fish, Oryzias latipes

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Author(s)

Abstract

A large insert genomic bacterial artificial chromosome (BAC) library was constructed from the inbred Hd-rR strain of the medaka, Oryzias latipes. Approximately 92, 000 clones were gridded on high-density replica filters. Insert analysis of randomly selected clones indicated a mean insert size of 210 kb and predicted a 24 times coverage of the medaka genome. The library was hybridized with a single locus DNA fragment, and the resulting positive clones were characterized and shown to be compatible with a 24-fold redundant library. This first large insert genomic library of the medaka should increase the speed of genomic analyses for this fish species.

Journal

  • Genes & Genetic Systems

    Genes & Genetic Systems 76(1), 61-63, 2001-02

    The Genetics Society of Japan

References:  14

Cited by:  7

Codes

  • NII Article ID (NAID)
    10006698255
  • NII NACSIS-CAT ID (NCID)
    AA11077421
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    13417568
  • NDL Article ID
    5716836
  • NDL Source Classification
    ZR1(科学技術--生物学)
  • NDL Call No.
    Z53-W539
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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