Stylonychia lemnaeの大核染色体DNAを利用した環状ベクターによる繊毛虫の形質転換〔英文〕

• ENDOH Hiroshi

  Institut für Biologie III, Abteilung Zellbiologie, Universität Tübingen

書誌事項

タイトル別名

• Transformation of ciliates with a circular plasmid derived from an overamplified macronuclear DNA of Stylonychia lemnae.
• Stylonychia lemnae ノ ダイ カク センショクタイ DNA

抄録

Transformation of ciliates was attempted with a circular plasmid containing a chromosomal DNA of the hypotrichous ciliate Stylonychia lemnae by calcium phosphate-mediated transfection. The plasmid, designated Tübingen-3, was constructed from a bacterial plasmid, a gene for neomycin resistance derived from a vector for the cellular slime mold Dictyostelium, and a 1.2 kb macronuclear chromosomal DNA of Stylonychia. Transformants were obtained at a frequency of one per 10⁴ to 10⁵ of input cells in Stylonychia lemnae and at a lower frequency, one per 10 ⁶, in Tetrahymena thermophila. The vector when simply linearized by digestion with restriction enzymes failed to give transformants, whereas when the vector was linearized so as to have short telomeric sequences at both ends, effective transformation was again observed. The Dictyostelium vector, pCERF DRpl4, with a segment of DNA that contained the putative origin of replication was found, unexpectedly, to be effective in transforming Stylonychia cells. The origin of replication in Stylonychia and Dictyostelium might thus be functionally similar.<br>

収録刊行物

• 遺伝学雑誌

  遺伝学雑誌 69 (4), 377-383, 1994

  日本遺伝学会

キーワード

• Genetics
• General Medicine

詳細情報

• CRID: 1390001205444307968
• NII論文ID: 10006701856
• NII書誌ID: AA00690924
• DOI: 10.1266/jjg.69.377
• ISSN: 18805787
• ISSN: 0021504X
• ISSN: http://id.crossref.org/issn/0021504X
• NDL書誌ID: 3895186
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I3895186
• Web Site: http://www.jstage.jst.go.jp/article/jjg/69/4/69_4_377/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可