Rolos of Mast Cells and Histamine in Mosquito Bite-Induced Allergic Itch-Associated Responses in Mice

• Ohtsuka Eiji

  Department of Applied Pharmacology, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University

• Kawai Sanae

  Department of Applied Pharmacology, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University

• Ichikawa Tomohiro

  Department of Applied Pharmacology, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University

• Nojima Hiroshi

  Department of Applied Pharmacology, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University

• Kitagawa Kanji

  Department of Applied Pharmacology, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University

• Shirai Yoshikazu

  Department of Biodefense Medicine, Faculty of Medicine, Toyama Medical and Pharmaceutical University

• Kamimura Kiyoshi

  Department of Biodefense Medicine, Faculty of Medicine, Toyama Medical and Pharmaceutical University

• Kuraishi Yasushi

  Department of Applied Pharmacology, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University

書誌事項

タイトル別名

• Roles of Mast Cells and Histamine in Mosquito Bite-Induced Allergic Itch-Associated Responses in Mice.
• Roles of mast cell and hista-mine in mosquito bite-induced allergic itch-associated responses in mice

抄録

We investigated itch-associated responses (scratching) to mosquito bites and the role of histamine and mast cells in mosquito-induced itching in mice. Although the first bites of mosquito Aedes albopictus did not increase scratching, repeated bites increased scratching. The response was not diminished even after an interval of 2 months. Similarly, repeated intradermal (i.d.) injections of salivary gland extract (SGE) from Aedes albopictus increased scratching after SGE injection itself and mosquito bites. The scratching peaked within 10 min and almost subsided by 60 min. The opioid antagonist naloxone (1 mg/kg, s.c.) inhibited scratching following SGE injection. Although the non-sedative H₁-histamine-receptor antagonist terfenadine (30 mg/kg, p.o.) significantly suppressed scratching induced by histamine (100 nmol/site, i.d.) in either naive or mosquito-sensitized mice, it did not affect mosquito-induced scratching in mosquito-sensitized mice. Repeated injections of SGE increased scratching in mast cell-deficient (WBB6F1-W/W^v) mice as well as in normal (WBB6F1-+/ +) littermates. Repeated exposure to mosquito bites roughly doubled serum concentrations of total IgE and IgG₁, but not IgG_2a. Repeated injections of SGE markedly increased plasma extravasation induced by mosquito bites and such an increase was almost completely suppressed by terfenadine (30 mg/kg, p.o.). The results show the presence of histamine-mediated and histamine-independent mechanisms in cutaneous itching and suggest that histamine probably released from mast cells does not play an important role in itching in immediate allergic reaction. Our murine model of mosquito itching may be useful for studying the mechanisms of immediate allergic itching.

収録刊行物

• Jpn.J.Pharmacol.

  Jpn.J.Pharmacol. 86 (1), 97-105, 2001

  公益社団法人 日本薬理学会

キーワード

• Immediate allergic itch
• Mosquito bite
• Itch-associated response
• Mast cell
• Histamine

詳細情報

• CRID: 1390282679261360256
• NII論文ID: 10007121733
• NII書誌ID: AA00691188
• DOI: 10.1254/jjp.86.97
• COI: 1:CAS:528:DC%2BD3MXktVWgu74%3D
• ISSN: 13473506
• ISSN: 00215198
• NDL書誌ID: 5786784
• PubMed: 11430478
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I5786784
• Web Site: https://api.elsevier.com/content/article/PII:S0021519819303920?httpAccept=text/xml
• Web Site: https://api.elsevier.com/content/article/PII:S0021519819303920?httpAccept=text/plain
• Web Site: https://search.jamas.or.jp/link/ui/2001250925
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可