導電性中空糸膜による糞便試料からの大腸菌O157の遺伝子抽出

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  • Gene-extraction of <i>Escherichia coli</i> O157 from feces by the metal coating hollow fiber membrane
  • ドウデンセイ チュウクウシ マク ニ ヨル フンベン シリョウ カラ ノ ダイチョウキン O157 ノ イデンシ チュウシュツ

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We have already developed the electrical sterilization method with the metal coating hollow fiber membrane. The sterilization mechanism was imagined that the cell membrane was broken by passing a direct current, because the leakage of intracellular contents was confirmed when bacteria were sterilized.<br>In this paper, the mechanism of this sterilization were focused and the possibility whether the metal coating hollow fiber membrane became a new device of gene extraction was examined. After the direct current to the membrane surface trapped Escherichia coli O157 was passed, the gene was checked. As a result, the vero toxin type 1-gene could be detected. The detection sensitivity on the gene extraction was mainly depended on the concentration of sodium dodecyl sulfate (SDS) which was an anion surfactant and the value of the direct current. The highest sensitivity in the extraction was obtained under 5% SDS and 300mA. In addition, the gene was extracted when 107 or 108 of Escherichia coli O157 were contained in 1ml of the real feces.<br>This system needed the time to extract the gene only in 10 minutes. The system was also simpler equipment which consists of the perista-pump and the power supply except the membrane.

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