Evaluation of PCR as a Means of Identification of Pasteurella pneumotropica.

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  • NOZU Ryoko
    ICLAS Monitoring Center, Central Institute for Experimental Animals
  • GOTO Kazuo
    ICLAS Monitoring Center, Central Institute for Experimental Animals
  • OHASHI Hiroaki
    ICLAS Monitoring Center, Central Institute for Experimental Animals
  • TAKAKURA Akira
    ICLAS Monitoring Center, Central Institute for Experimental Animals
  • ITOH Toshio
    ICLAS Monitoring Center, Central Institute for Experimental Animals

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A polymerase chain reaction with new primers (new PCR) designed from Pasteurella pneumotropica 16S rDNA as an identification system for this organism was compared with the PCR reported by Wang et al. (Wang's PCR) by using 15 bacterial reference species and 70 clinical isolates with the conventional identification system. For the 15 reference strains, both PCRs were identical. For the 70 clinical isolates, the new PCR and Wang's PCR showed consistency with the conventional system in 62.9% (44/70) and 51.4% (36/70), respectively. Twenty-six isolates were inconsistent with the conventional system and the new PCR with respect to morphology and serology. These findings suggested that the new PCR was more sensitive than Wang's PCR, and the new PCR in combination with morphology and serology is useful for P. pneumotropica identification.<br>

収録刊行物

  • Experimental Animals

    Experimental Animals 48 (1), 51-54, 1999

    公益社団法人 日本実験動物学会

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