Role of Adenosine in Renal Protection Induced by a Brief Episode of Ischemic Preconditioning in Rats.

• Sugino Haruko

  Division of Pathophysiology, Center for Clinical Pharmacy and Clinical Sciences, School of Pharmaceutical Sciences, Kitasato University

• Shimada Hideyo

  Division of Pathophysiology, Center for Clinical Pharmacy and Clinical Sciences, School of Pharmaceutical Sciences, Kitasato University

• Tsuchimoto Kanji

  Division of Pathophysiology, Center for Clinical Pharmacy and Clinical Sciences, School of Pharmaceutical Sciences, Kitasato University Kitasato Institute Hospital

抄録

The protective effect of a brief episode of ischemic preconditioning was examined at an early phase of ischemic-reperfusion injury in the rat kidney. Rats were subjected to 50 min of left renal artery occlusion followed by 120 min of reperfusion. Ischemic preconditioned rats were subjected to preconditioning with two cycles of 3-min ischemia and 5-min reperfusion (IPC). Ischemic-reperfusion injury led to a low recovery of the glomerular filtration rate (GFR). Overt morphological changes, consisting of blood trapping and tubular collapse, were seen. IPC improved the recovery of GFR and renal morphology. The IPC effect was not blocked by 8-(p-sulfophenyl)-theophylline (SPT), a non-selective adenosine receptor antagonist, by 1,3-dipropyl-8-cyclopentylxanthine (DPCPX), a selective A₁-receptor antagonist, or by 3,7-dimethyl-1-propargylxanthine (DMPX), a selective A₂-receptor antagonist. Intravenous infusion of adenosine (30 μg/min per rat, for 5 min) prior to the 50-min occlusion improved the recovery of GFR, and this protection of GFR was blocked by SPT. Thus, both IPC and exogenous adenosine attenuated ischemic-reperfusion injury of the kidney. However, because three adenosine receptor antagonists failed to abolish the protective effect of IPC, there is no evidence to indicate that activation of adenosine receptors contributes to the IPC effect in the kidney.

収録刊行物

• Jpn.J.Pharmacol.

  Jpn.J.Pharmacol. 87 (2), 134-142, 2001

  公益社団法人 日本薬理学会

キーワード

• Acute renal failure
• Ischemia
• Reperfusion
• Preconditioning
• Adenosine

詳細情報

• CRID: 1390001204287554304
• NII論文ID: 10007368147
• NII書誌ID: AA00691188
• DOI: 10.1254/jjp.87.134
• COI: 1:CAS:528:DC%2BD3MXnvF2qurc%3D
• ISSN: 13473506
• ISSN: 00215198
• NDL書誌ID: 5950634
• PubMed: 11700012
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I5950634
• Web Site: https://api.elsevier.com/content/article/PII:S0021519819302860?httpAccept=text/xml
• Web Site: https://api.elsevier.com/content/article/PII:S0021519819302860?httpAccept=text/plain
• Web Site: https://search.jamas.or.jp/link/ui/2002131145
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可