One-tube post-PCR fluorescent labeling of DNA fragments.

• M Inazuka

• T Tahira

• K Hayashi

Abstract

A method for fluorescent postlabeling of PCR products has been developed. The method uses Klenow fragment of DNA polymerase I that exchanges the 3'-terminal residue of PCR-amplified DNA fragment for fluorescent nucleotides. All reactions, including PCR, are performed in one tube simply by successive addition of reagents. The products can be applied directly to fluorescence-based automated DNA sequencers without purification for either length determination in denaturing electrophoresis or mutation detection in SSCP electrophoresis.

Journal

• Genome Research

  Genome Research 6 (6), 551-557, 1996-06

  Cold Spring Harbor Laboratory

Keywords

• Genetics (clinical)
• Genetics

Details

• CRID: 1360855570497809536
• NII Article ID: 10007570100
• NII Book ID: AA11054029
• DOI: 10.1101/gr.6.6.551
• ISSN: 10889051
• ISSN: http://id.crossref.org/issn/10889051
• PubMed: 8828044
• Web Site: https://syndication.highwire.org/content/doi/10.1101/gr.6.6.551
• Data Source:
  • Crossref
  • PubMed
  • CiNii Articles
  • LSDB Archive