Effect of Aggregation of Amphotericin B on Lysophosphatidylcholine Micelles as Related to Its Complex Formation with Cholesterol or Ergosterol.
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- Kawabata Manami
- Department of Environmental Sciences, Faculty of Science, Osaka Women's University
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- Onda Maki
- Department of Environmental Sciences, Faculty of Science, Osaka Women's University
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- Mita Tomoyoshi
- Department of Environmental Sciences, Faculty of Science, Osaka Women's University
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The effect of aggregation of amphotericin B (AmB), as well as the complex formation of AmB with cholesterol or ergosterol, was investigated in micelles and vesicles. AmB in lysophosphatidylcholine (LPC) micelles adopted a more favorable monomeric form than that in other drug formulations. At an LPC/AmB ratio of 200, AmB existed only in monomeric form. Such monomeric behavior is likely dependent upon the fluidity and size of the micelles. In LPC micelles composed of 90% monomeric AmB, AmB-ergosterol complex formation occurred with an increase in the sterol concentration, but the complex formation of AmB-cholesterol was slight. On the other hand, in LPC micelles composed of 40% monomeric AmB, the complex formation of AmB-cholesterol as well as AmB-ergosterol was extensive. These results suggest that the complex formation of AmB with both sterols is highly dependent upon the aggregated state of AmB. In addition, using monolayers, mixtures of AmB/LPC/ergosterol were became more stable with rising temperature, while the stability of mixtures of AmB/LPC/cholesterol remained unchanged, implying that complex formation of AmB with cholesterol is different from that of AmB with ergosterol.
収録刊行物
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- The Journal of Biochemistry
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The Journal of Biochemistry 129 (5), 725-732, 2001
社団法人 日本生化学会
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詳細情報 詳細情報について
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- CRID
- 1390282679908274176
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- NII論文ID
- 130003534137
- 10007855295
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- NII書誌ID
- AA00694073
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- COI
- 1:CAS:528:DC%2BD3MXltVWltbo%3D
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- ISSN
- 17562651
- 0021924X
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- NDL書誌ID
- 5752578
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- PubMed
- 11328594
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可