新たに発見された有機水銀分解遺伝子merB3とその発現調節系を利用した有機水銀の検出方法に関する研究  [in Japanese] Study on the Organomercury Detection Method Using a New Organomercury Lyase Gene, merB3, and a Regulation System of the Gene Expression  [in Japanese]

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Author(s)

    • 山肩 健史 YAMAGATA Takeshi
    • 東北学院大学工学部環境生物工学研究室 Laboratory of Environmental Biotechnology, Faculty of Eng., Tohoku Gakuin University
    • 石井 秀学 ISHII Hidenori
    • 東北学院大学工学部環境生物工学研究室 Laboratory of Environmental Biotechnology, Faculty of Eng., Tohoku Gakuin University
    • 成田 勝 [他] NARITA Masaru
    • 東北学院大学工学部環境生物工学研究室 Laboratory of Environmental Biotechnology, Faculty of Eng., Tohoku Gakuin University
    • 熊谷 康 KUMAGAI Yasushi
    • 東北学院大学工学部環境電磁研究室ElectromagneticEnvironmental Laboratory,Faculty of Eng., Tohoku Gakuin University Electromagnetic Environmental Laboratory, , Faculty of Eng., Tohoku Gakuin University
    • 遠藤 銀朗 ENDO Ginro
    • 東北学院大学工学部環境生物工学研究室 Laboratory of Environmental Biotechnology, Faculty of Eng., Tohoku Gakuin University

Abstract

Mercurials have been released into the environment by geological or anthropogenic activities. Organomercurial compounds such as methylmercury are known as toxic compounds. Therefore, organomercurials should be monitored to keep clean environments. However, chemical species of organomercurials are not easily detected separately or individually.<br>In this study, we tried to detect organomercurial compounds by using a bacterial gene-expression system. A series of <i>mer</i> operon-luciferase (<i>mer-lux</i>) transcriptional fusion plasmids (pHYΔB3Lux and pHYB3Lux) was constructed to evaluate the gene expression system with a new organomercury lyase gene <i>merB3</i> from <i>Bacillus megaterium</i> MB1. A plasmid (pGR1A) encoding <i>mer</i> operon genes from O/P<sub>merR1</sub> to <i>merA</i> isolated from <i>B. megaterium</i> MB1 was used as a transacting gene expression vector with the <i>mer-lux</i> transcriptional fusion plasmids into a same host bacterial cell. The transformants that carry a set of two plasmids (pHYΔB3Lux+pGR1A or pHYB3Lux+pGR1A), respectively) were used for detection of organomercurials. The experimental results showed that the transformant with pHYΔB3Lux+pGR1A responded to only mercury chloride (MC). On the other hand, the transformant with pHYB3Lux+pGR1A responded and detected MC and the all organomercurials tested in the study. Therefore, the bacterial strain which possess a gene expression system of <i>mer-lux</i> transcriptional fusion was useful to detect organomercurials with distinction from inorganic mercury.

Journal

  • Journal of Japan Society on Water Environment

    Journal of Japan Society on Water Environment 24(4), 219-224, 2001-04-10

    Japan Society on Water Environment

References:  10

Codes

  • NII Article ID (NAID)
    10008004805
  • NII NACSIS-CAT ID (NCID)
    AN10372439
  • Text Lang
    JPN
  • Article Type
    ART
  • ISSN
    09168958
  • NDL Article ID
    5739302
  • NDL Source Classification
    ZN5(科学技術--建設工学・建設業--都市工学・衛生工学)
  • NDL Call No.
    Z16-1045
  • Data Source
    CJP  NDL  J-STAGE 
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