エンドヌクレアーゼ・センシティブ・サイト法によるEscherichia coliの紫外線不活化および光回復の評価  [in Japanese] UV Inactivation and Photoreactivation of Escherichia coli Determined by Endonuclease Sensitive Site Assay  [in Japanese]

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Author(s)

Abstract

Ultraviolet (UV) light inactivation of <i>Escherichia coli</i> was investigated with the endonuclease sensitive site (ESS) assay which can detect the UV-induced pyrimidine dimers in the genomic DNA. High correlation was observed between the UV dose irradiated for <i>E.coli</i> and the number of ESS induced in the DNA of <i>E.coli</i> (r<sup>2</sup>=0.996). The survival ratio of <i>E.coli</i> also showed high correlation with the number of ESS induced in the DNA (r<sup>2</sup>=0.999). Photoreactivation of <i>E.coli</i> after 99.9% inactivation by UV irradiation was also investigated with ESS assay. During the exposure to fluorescent light, ESS in the DNA of <i>E.coli</i> were continuously repaired while the survival ratio of them gradually recovered. The number of ESS was correlated with the survival ratio of <i>E.coli</i> during fluorescent light irradiation (r<sup>2</sup>=0.937), indicating that the repair of pyrimidine dimers in the genomic DNA would contribute to the survival recovery of <i>E.coli</i> during photoreactivation. It was indicated, in this study, that ESS assay was useful to investigate UV inactivation and photoreactivation of <i>E.coli</i> quantitatively.

Journal

  • Journal of Japan Society on Water Environment

    Journal of Japan Society on Water Environment 24(8), 534-538, 2001-08-10

    Japan Society on Water Environment

References:  17

Cited by:  1

  • 光放射の応用  [in Japanese]

    河本 康太郎 , 佐々木 政子 , 白水 俊次 , 田澤 信二 , 戸田 淨 , 谷口 正成 , 木下 忍 , 田中 總太郎 , 木村 嘉孝

    Journal of the Illuminating Engineering Institute of Japan 86(8), 649-658, 2002-08-01

    References (285)

Codes

  • NII Article ID (NAID)
    10008005484
  • NII NACSIS-CAT ID (NCID)
    AN10372439
  • Text Lang
    JPN
  • Article Type
    Journal Article
  • ISSN
    09168958
  • NDL Article ID
    5882749
  • NDL Source Classification
    ZN5(科学技術--建設工学・建設業--都市工学・衛生工学)
  • NDL Call No.
    Z16-1045
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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