遺伝子組換え酵母を用いた各種排水中の女性ホルモン様活性の測定  [in Japanese] Evaluation of Estrogen-like Activity in Wastewater Using Recombinant Yeast  [in Japanese]

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Author(s)

    • 宮 晶子 MIYA Akiko
    • 株式会社荏原総合研究所技術研究センター Technology Research Center, Ebara Research Co., Ltd.
    • 葛 甬生 [他] KATSU Yosei
    • 株式会社荏原製作所エンジニアリング事業本部水環境プロセス開発室 Water Environmental Process Development Dept., Environmental Engineering Group, Ebara Corporation.
    • 田中 俊博 TANAKA Toshihiro
    • 株式会社荏原製作所エンジニアリング事業本部水環境プロセス開発室 Water Environmental Process Development Dept., Environmental Engineering Group, Ebara Corporation.

Abstract

Various wastewater treatment plants located in Japan were evaluated for investigating behavior of estrogenic substances using <i>in vitro</i> recombinant yeast assay, enzyme immunoassay (EIA) and chemical analysis. Expressed in 17β-estradiol (E2) equivalent, total estrogen-like activities in influents were found 0.099μg·<i>l</i><sup>-1</sup> in sewage, 4.0μg·<i>l</i><sup>-1</sup> in treating animal wastewater and 0.25μg·<i>l</i><sup>-1</sup> in landfill leachate. The results indicated that 17β-estradiol (E2) (analyzed by EIA) contributed to estrogen-like activity (analyzed by yeast assay) in influents and treated effluents of sewage and treating animal wastewater. On the other hand, bisphenol A significantly contributed to estrogen-like activity in landfill leachate. The activities by yeast assay in some influents of sewage exceeded E2 concentration considerably, suggesting the presence of an unknown estrogenic substance. In these cases, it was found that sewage sample have the overestimation effect on the estrogen-like activity of E2. According these results, it is necessary to evaluate of estrogen-like activity in wastewater to consider effects of some contaminants in it.

Journal

  • Journal of Japan Society on Water Environment

    Journal of Japan Society on Water Environment 24(11), 750-756, 2001-11-10

    Japan Society on Water Environment

References:  12

Cited by:  3

Codes

  • NII Article ID (NAID)
    10008006007
  • NII NACSIS-CAT ID (NCID)
    AN10372439
  • Text Lang
    JPN
  • Article Type
    Journal Article
  • ISSN
    09168958
  • NDL Article ID
    5975857
  • NDL Source Classification
    ZN5(科学技術--建設工学・建設業--都市工学・衛生工学)
  • NDL Call No.
    Z16-1045
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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