酵母Two-hybrid法を用いた環境試料のエストロゲン様活性評価に関する研究  [in Japanese] Modified Yeast Two-hybrid Assay to Evaluate the Estrogenic Activity of Environmental Samples  [in Japanese]

Access this Article

Search this Article

Author(s)

Abstract

A number of bioassays for screening the compounds for estrogenic activity have been proposed so far, however, a yeast two-hybrid system appears more simple and inexpensive assay with high repeatability. The biggest problem of this bioassay is that it cannot measure estrogenic activity in some of the environmental samples. At is was assumed that the co-existing compounds were responsible for these false negatives, two methods were tested:<br>1. Separation of such co-existing chemical interferences by use of liquid chromatographic method.<br>2. Masking the retarding effect of such co-existing chemicals interferences by the addition of S9mix.<br>The results indicated that both methods were effective to identify the estrogenic activity, however, the addition of S9mix was much more simple to conduct and more effective to determine the estrogenic activity in environmental samples.

Journal

  • Journal of Japan Society on Water Environment

    Journal of Japan Society on Water Environment 24(12), 865-870, 2001-12-10

    Japan Society on Water Environment

References:  9

Cited by:  5

Codes

  • NII Article ID (NAID)
    10008006225
  • NII NACSIS-CAT ID (NCID)
    AN10372439
  • Text Lang
    JPN
  • Article Type
    Journal Article
  • ISSN
    09168958
  • NDL Article ID
    6007402
  • NDL Source Classification
    ZN5(科学技術--建設工学・建設業--都市工学・衛生工学)
  • NDL Call No.
    Z16-1045
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
Page Top