フェナントロリンコバルト錯体をメディエーターとするD-フルクトースデヒドロゲナーゼを用いたフルクトースバイオセンサ Fructose Biosensor Based on D-fructose Dehydrogenase and Phenanthroline Cobalt Complex as a Mediator

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Abstract

<p>Fructose biosensor based on D-Fructose dehydrogenase (FDH) was developed with use of tris (1,10-phenanthroline) cobalt (II) complex (Co(phen)<sub>3</sub><sup>2+</sup>) as a redox mediator. Co(phen)<sub>3</sub><sup>2+</sup> could be prepared easily and was oxidized reversibly at low potential. For the immobilization of FDH, a gold electrode was modified through chemisorption of cystamine. FDH was covalently bound to self-assembled monolayer of cystamine by cross-linking amino groups of cystamine and FDH molecule using glutaraldehyde. With use of Co(phen)<sub>3</sub><sup>2+</sup> as a redox mediator, the catalytic current of the FDH-modified gold electrode increased by the addition of D-fructose. In the immobilization procedure, 0.2 mg/ml of FDH resulted in the largest catalytic response among other concentration of FDH. The best sensitivity of the sensor was obtained at pH 7.0 and 35°C. The calibration curve exhibits a linear relationship between 10 and 110 µM with a correlation coefficient of 0.971 and the relative standard deviation of 0.57% (<i>n</i> = 3).</p>

Journal

  • Electrochemistry

    Electrochemistry 70(4), 258-263, 2002-04-05

    The Electrochemical Society of Japan

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Codes

  • NII Article ID (NAID)
    10008009506
  • NII NACSIS-CAT ID (NCID)
    AN00151637
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    13443542
  • NDL Article ID
    6128144
  • NDL Source Classification
    ZP1(科学技術--化学・化学工業)
  • NDL Call No.
    Z17-14
  • Data Source
    CJP  NDL  J-STAGE 
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