導電性中空糸膜による大腸菌O 157からのベロ毒素遺伝子の抽出  [in Japanese] Extraction of the VT1-gene from Escherichia coli O 157 by the metal coationg hollow fiber membrane  [in Japanese]

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Author(s)

Abstract

We have already developed the electrical sterilization method with the metal coating hollow fiber membrane. The sterilization mechanism was imagined as follows : the leakage of intracellular contents was confirmed when bacteria were sterilized by a direct electric current. This leakage proved indirectly that the cell membrane was partially broken.<BR>In this paper, the leakage phenomenon and the mechanism on the sterilization were focused and the possibility whether the metal coating hollow fiber membrane became a new device of gene extraction was examined. After the direct electric current to the membrane surface trapped <I>Escherichia coli</I> O 157 was passed, the gene was checked. As a result, the vero toxin type 1-gene could be detected. The detection sensitivity on the gene extraction was mainly depended on the concentration of sodium dodecyl sulfate (SDS) which was an anion surfactant and the value of the direct electric current. The highest sensitivity in the extraction was obtained under 5 % SDS and 300 mA. In addition, the gene was extracted under <I>Escherichia coli</I> O 157 of 10<SUP>7</SUP>or 10<SUP>8</SUP>CFU/m<I>l</I> contained in other bacteria of 10<SUP>8</SUP>CFU/m<I>l</I>.<BR>This system needed the time to extract the gene only in 10 minutes. The system was also simpler equipment which consists of the perista-pump and the power supply except the membrane.

Journal

  • MEMBRANE

    MEMBRANE 26(1), 46-51, 2001-01-01

    THE MEMBRANE SOCIETY OF JAPAN

References:  15

Cited by:  1

Codes

  • NII Article ID (NAID)
    10008010265
  • NII NACSIS-CAT ID (NCID)
    AN0023215X
  • Text Lang
    JPN
  • Article Type
    Journal Article
  • ISSN
    03851036
  • NDL Article ID
    5660191
  • NDL Source Classification
    ZR2(科学技術--生物学--生化学)
  • NDL Call No.
    Z18-1127
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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