ナノ分子集合体逆ミセルの生物工学的応用  [in Japanese] Novel Biological Application of Nanostructured Reversed Micelles  [in Japanese]

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Author(s)

    • 後藤 雅宏 GOTO Masahiro
    • 九州大学大学院工学研究院応用化学部門 Department of Applied Chemistry, Graduate School of Engineering, Kyushu University

Abstract

Reversed micelles create unique environment in organic media. They are capable of solubilizing hydrophilic biomolecules (e.g., proteins, peptides, amino acids, DNA) in their aqueous interior. This feature brings about the practical use of biomaterials in organic media because reversed micelles can solubilize them preserving their intrinsic activity. In this article, we focus on novel biological application of reversed micelles : purification of biomolecules (proteins and DNAs), protein renaturation, and the detection of unusual sequence of DNAs. In the reversed micellar phase, denatured proteins are completely reactivated under optimum conditions. This novel refolding technique facilitates a high renaturation yield at high protein concentration. Recently, the reversed micelle is found to be a useful tool not only for protein separation but also for protein renaturation. Furthermore, we achieved the extraction of DNAs from an aqueous solution into isooctane using reversed micelles. The degree of extraction of DNAs was strongly influenced by the type of surfactant in the organic solvent and ionic strength in the aqueous phase. The driving force of the DNA transfer was electrostatic interaction between cationic surfactants and the negatively charged DNA. Cationic surfactants possessing two long-alkyl chains are the best candidate for the successful transfer of DNAs from the aqueous solution to the reversed micellar solution. We also succeeded in detecting the targeted DNAs that have a missmatched sequence by utilizing a reversed micellar solution.

Journal

  • MEMBRANE

    MEMBRANE 26(4), 179-184, 2001-07-01

    THE MEMBRANE SOCIETY OF JAPAN

References:  17

Cited by:  1

  • Extraction・Liquid Membrane  [in Japanese]

    大島 達也 , 後藤 雅宏 , 久保田 富生子

    化学工学 66(10), 589-590, 2002-10-05

    References (26)

Codes

  • NII Article ID (NAID)
    10008010730
  • NII NACSIS-CAT ID (NCID)
    AN0023215X
  • Text Lang
    JPN
  • Article Type
    Journal Article
  • ISSN
    03851036
  • NDL Article ID
    5858118
  • NDL Source Classification
    ZR2(科学技術--生物学--生化学)
  • NDL Call No.
    Z18-1127
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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