逆ミセルを用いる遺伝子変異の検出  [in Japanese] Mutation Detection of DNA by Reversed Micelles  [in Japanese]

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Author(s)

Abstract

Reversed micelles, which are nanostructural molecular assemblies, are utilized for the mutation detection of deoxyribonucleic acids (DNAs). The hybridization rate of a complementary single stranded DNA was measured in reversed micelles by detecting the decrease in the absorbance at 260nm with a UV/VIS spectrophotometer. The hybridization rate was significantly reduced when the complementary DNA has a mismatched sequence. The number and the position of the mutation also influenced the hybridization rate in reversed micelles. Therefore, we can readily know the existence of a mutation for a targeted DNA by measuring the hybridization rate. The analysis of sample oligonucleotides (20mer) that relates to the tumor suppressor gene P53 was conducted in the AOT reversed micelle. The hybridization rate was very sensitive to the number and the location of the mismatched sequence in the sample DNAs.

Journal

  • MEMBRANE

    MEMBRANE 27(2), 89-93, 2002-03-01

    THE MEMBRANE SOCIETY OF JAPAN

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Codes

  • NII Article ID (NAID)
    10008011213
  • NII NACSIS-CAT ID (NCID)
    AN0023215X
  • Text Lang
    JPN
  • Article Type
    Journal Article
  • ISSN
    03851036
  • NDL Article ID
    6123808
  • NDL Source Classification
    ZR2(科学技術--生物学--生化学)
  • NDL Call No.
    Z18-1127
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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