膜タンパク質の膜間移行現象を利用した人工膜ワクチン調製法の検討

• 横田 賢

  富山医科薬科大学薬学部

• 玉木 慎也

  富山医科薬科大学薬学部

• 林 京子

  富山医科薬科大学医学部

• 上野 雅晴

  富山医科薬科大学薬学部

書誌事項

タイトル別名

• Basic Study on Development of Artificial Membrane Vaccine Using Inter-membrane Protein Transfer.
• マク タンパクシツ ノ マク カン イコウ ゲンショウ オ リヨウ シタ ジンコウ マク ワクチン チョウセイホウ ノ ケントウ

抄録

Membrane protein transfer from influenza virus-infected cells to liposomes was examined in several experimental conditions using gel-filtration, SDS PAGE and fluorescent microscopy. In gel-filtration it was found that membrane protein spontaneously transferred from cell membrane to liposomal membrane during incubation of liposomes with cells, accompanied by some leaks of non-membrane protein.The amount of membrane protein incorporated into liposomal membrane was proportional to that of total protein released from cells. SDS PAGE experiment after immunoprecipitation confirmed the transfer of influenza antigenic protein from influenza virus-infected cell's membrane to liposomal membrane. Fluorescent image was observed all around liposome surface in microscopy using fluorescence antibody, suggesting the transferred antigenic protein was incorporated throughout into liposomal membrane.

収録刊行物

• 膜

  膜 27 (2), 94-100, 2002

  日本膜学会

キーワード

• liposome
• protein transfer
• membrane protein
• influenza virus

詳細情報

• CRID: 1390282681399830016
• NII論文ID: 10008011223
• NII書誌ID: AN0023215X
• DOI: 10.5360/membrane.27.94
• ISSN: 18846440
• ISSN: 03851036
• NDL書誌ID: 6123831
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6123831
• Web Site: https://search.jamas.or.jp/link/ui/2002165648
• 本文言語コード: ja
• データソース種別:
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  • NDL
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• 抄録ライセンスフラグ: 使用不可