Impatiens necrotic spot virus によるシクラメンえそ斑紋病およびエキザカムえそ斑紋病  [in Japanese] Necrotic Spot Disease of Cyclamen (Cyclamen persicum) and Exacum (Exacum affine) Caused by Impatiens Necrotic Spot Virus (INSV)  [in Japanese]

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Author(s)

Abstract

Viral symptoms with necrotic spots and necrotic ring spots were found in cyclamen (<i>Cyclamen persicum</i>) and exacum (<i>Exacum affne</i>) plants in Tochigi prefecture. The double-antibody sandwich enzyme-linked immuno-absorbent assay with antiserum to <i>Tomato spotted wilt virus</i> (TSWV) gave a negative reaction. Spherical enveloped particles were observed with electron microscopy in infected cyclamen leaves. Degenerate primers specific to the nucleocapsid genes of <i>Impatiens necrotic spot virus</i> (INSV) and TSWV were designed for reverse transcription-polymerase chain reaction (RT-PCR). RT-PCR gave the expected PCR products of approximately 540bp and 440bp. Sequence comparison of the PCR products showed 99.1% and 98.8% homology with INSV (NL-07) in nucleotide and amino acid, respectively, indicating that the causal agent of necrotic spot diseases of cyclamen and exacum was INSV.

Journal

  • Annual Report of the Kanto-Tosan Plant Protection Society

    Annual Report of the Kanto-Tosan Plant Protection Society 48, 97-100, 2001-11-01

    The Kanto-Tosan Plant Protection Society

References:  6

Cited by:  2

Codes

  • NII Article ID (NAID)
    10008014645
  • NII NACSIS-CAT ID (NCID)
    AN00048332
  • Text Lang
    JPN
  • Article Type
    Journal Article
  • ISSN
    03888258
  • NDL Article ID
    6167711
  • NDL Source Classification
    ZR7(科学技術--農林水産--農産)
  • NDL Call No.
    Z18-120
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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