Impatiens necrotic spot virus によるシクラメンえそ斑紋病およびエキザカムえそ斑紋病 [in Japanese] Necrotic Spot Disease of Cyclamen (Cyclamen persicum) and Exacum (Exacum affine) Caused by Impatiens Necrotic Spot Virus (INSV) [in Japanese]
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Viral symptoms with necrotic spots and necrotic ring spots were found in cyclamen (<i>Cyclamen persicum</i>) and exacum (<i>Exacum affne</i>) plants in Tochigi prefecture. The double-antibody sandwich enzyme-linked immuno-absorbent assay with antiserum to <i>Tomato spotted wilt virus</i> (TSWV) gave a negative reaction. Spherical enveloped particles were observed with electron microscopy in infected cyclamen leaves. Degenerate primers specific to the nucleocapsid genes of <i>Impatiens necrotic spot virus</i> (INSV) and TSWV were designed for reverse transcription-polymerase chain reaction (RT-PCR). RT-PCR gave the expected PCR products of approximately 540bp and 440bp. Sequence comparison of the PCR products showed 99.1% and 98.8% homology with INSV (NL-07) in nucleotide and amino acid, respectively, indicating that the causal agent of necrotic spot diseases of cyclamen and exacum was INSV.
- Annual Report of the Kanto-Tosan Plant Protection Society
Annual Report of the Kanto-Tosan Plant Protection Society 48, 97-100, 2001-11-01
The Kanto-Tosan Plant Protection Society