宿主昆虫への Steinernema glaseri の誘引および選択行動の試験モデル Experimental Models for Testing Attraction and Preferential Behaviour of Steinernema glaseri to Several Insects

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Author(s)

    • 近藤 栄造 KONDO Eizo
    • Department of Applied Biological Sciences, Faculty of Agriculture, Saga University Department of Applied Biological Sciences, Faculty of Agriculture, Saga University
    • 吉賀 豊司 YOSHIGA Toyoshi
    • Department of Applied Biological Sciences, Faculty of Agriculture, Saga University Department of Applied Biological Sciences, Faculty of Agriculture, Saga University

Abstract

ペトリ皿を用いた行動解析試験結果に基づき、<I>Steinernema glaeri</I>感染態幼虫の誘引行動と選択性のモデルを提唱した。前者では、宿主昆虫から発散する誘引物質の有無に基づく線虫の分布確率をスコアー化し、宿主に到達した線虫数に重みづけを行い、その結果に基づいて、線虫の誘引行動を数量的に解析した。後者では、4種昆虫 (ハチミツガ、ハスモンヨトウ、チャバネゴキブリ、トノサマバッタ) への線虫の選択性を試験した。誘引源への線虫の誘引率は時間とともに高くなり、線虫接種6時間後には最高 (67.6%) に達した。ハチミツガ幼虫の摩砕液への誘引率は、摩砕液の濃度に依存した。誘引試験ならびに4種昆虫を用いた選択試験の結果、<I>S. glaseri</I>感染態幼虫の選好性は、ハチミツガ幼虫に対して最も強かった。

Two experimental models, one for testing the attraction behaviour (Petri-dish scoring system) and the other for analyzing preferential behaviour (preference test chambers) of infective juveniles of <I>Steinernema glaseri</I> are described and proposed. Petri-dish scoring system was based on the probability of nematode distribution in the presence and absence of the insects. It was given weightage, depending upon the number of nematodes reaching to the insects. The weighted responses were given ranks and scores to make quantitative analysis of the attraction behaviour of nematodes. The preference test chambers were designed to test preferential behaviour of the nematodes for the larvae of four insect species: <I> Galleria mellonella, Spodoptera litura, Blattella germanica</I> and <I>Locust migratoria</I>. The attraction rates of <I>S. glaseri</I> increased with the time and reached to maximum (67.6%) 6 hr after inoculation of the nematodes. <I>S. glaseri</I> responded to homogenate of <I>G. mellonella</I> larvae in a dose dependent manner. Maximum values for the absolute and relative preferential indices showed that the nematodes preferred <I>G. mellonella</I> most among the four insects examined.<I>Jpn. J. Nematol.</I> 30 (1/2): 35-46 (2000).

Journal

  • Nematological Research (Japanese Journal of Nematology)

    Nematological Research (Japanese Journal of Nematology) 30(1), 35-46, 2000-12-28

    The Japanese Nematological Society

References:  10

Codes

  • NII Article ID (NAID)
    10008014879
  • NII NACSIS-CAT ID (NCID)
    AN10453166
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    09196765
  • NDL Article ID
    5728818
  • NDL Source Classification
    ZR4(科学技術--生物学--動物)
  • NDL Call No.
    Z18-1311
  • Data Source
    CJP  NDL  J-STAGE  JASI 
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