Effects of L-Ascorbic Acid and Superoxide Anion Radical on the Polymerization of Ovalbumin

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Abstract

L-Ascorbic acid (AsA) plays an important role in food, especially in the rheological properties of bread dough. So in this study, we estimated the effect of AsA on the polymerization of proteins using ovalbumin (OVA). OVA was separated into 4 fractions by size-exclusion high-performance liquid chromatography (SE-HPLC) on the basis of molecular weight. When the OVA was incubated at 55°C, the relative areas of fraction 1 (molecular weight of more than 350,000) increased. The relative area of fraction 1 of OVA added with AsA, and of the OVA with superoxide anion radical (O<sub>2</sub><sup>−</sup>) generating system was increased. Furthermore, the remaining sulfhydryl group content in OVA with the O<sub>2</sub><sup>−</sup> generating systems showed a significant decrease from control. Thus, it was suggested that the O<sub>2</sub><sup>−</sup> produced during the autoxidation of AsA might react with the sulfhydryl groups, and consequently affect the polymerization of OVA.<br>

Journal

  • Food Science and Technology Research

    Food Science and Technology Research 7(3), 220-223, 2001-08-01

    Japanese Society for Food Science and Technology

References:  32

Codes

  • NII Article ID (NAID)
    10008015944
  • NII NACSIS-CAT ID (NCID)
    AA11267620
  • Text Lang
    ENG
  • Article Type
    NOT
  • ISSN
    13446606
  • Data Source
    CJP  J-STAGE 
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