ガザミ自然集団におけるミトコンドリアDNA-Dループ領域の制限酵素切断型多型  [in Japanese] Genetic Variability of Swimming Crab Based on PCR-RFLP Analysis of Mitochondrial DNA D-Loop Region  [in Japanese]

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Abstract

ガザミ8標本群, 合計234個体について集団内と集団間の遺伝的変異性および集団構造を把握する目的でミトコンドリアDNAのDループ領域を4種類の制限酵素で分析した。その結果, 集団内の遺伝的多様性を示すハプロタイプ多様度<I>h</I>と塩基多様度π値はそれぞれ0.403 (宮城県) ~0.903 (岡山県) , 0.0020 (宮城県) ~0.0101 (静岡県) となり, 宮城県の標本群では両方の値が低く遺伝的変異性が低いことが示された。また, これらの値と既報の全領域の結果と比較した結果, <I>h</I>では大きく異ならないがπ値では全領域の結果の約3.9倍に達し, 多型性がより明確に検出できると考えられた。宮城県と熊本県の標本群の<I>h</I>とπ値について, 近縁のノコギリガザミ類をも含めて検討した結果, どの種よりも明らかに低い値を示し, πでは顕著であった。<BR>標本群間の遺伝的異質性を比較した結果, 宮城県が他のすべての標本群との間に異質性が認められ, さらに熊本県は静岡県や岡山県と異なり, 分布の両端にあるこれらの2集団は他の地方集団と明らかに異なった遺伝的組成をもつことが示された。<BR>本研究では日本各地のガザミ地域集団の遺伝的変異性, 集団間の分化を示し, さらに人工種苗放流が自然集団に及ぼす人為的影響についても論議した。

Restriction fragment length polymorphism analysis was performed on Polymerase Chain Reaction amplified DNA fragments containing the D-loop region of swimming crab <I>Portunus trituberculatus</I> mitochondrial DNA (mtDNA) . A total of 234 individuals consisting of eight populations around Japan were surveyed with four restriction endonucleases (<I>Dra</I> I, <I>Hinc</I> II, <I>Mbo</I> I and <I>Psh</I>B I), yielding a total of 28 composite haplotypes. Within population of haplotypic diversity (<I>h</I>) and nucleotide diversity (π) were ranging from 0.403 (Miyagi) to 0.903 (Okayama) and from 0.0020 (Miyagi) to 0.0101 (Shizuoka), respectively. The Miyagi population was highly distinct from all other populatons. The homogeneity test by a chi-square randomization analysis demonstrated significant differences between Miyagi population and all the other six populations examined, and between Kumamoto and other two populations, Sizuoka and Okayama. The dendrogram clustering based on nucleotide divergence between populations, showed no clustering group of georaphical cline. Significance of hapolotype diversity (<I>h</I>) and nucleotide diversity (π) values obtained of swimming crab populations in the present study were discussed together with the data in mud crabs, differences in the genetic diversity between the mtDNA whole region and D-loop region, and the effects of stocking of artificially propagated larvae to the genetic composition of local populations in the swimming crab.

Journal

  • Aquaculture Science

    Aquaculture Science 50(1), 1-7, 2002-03-25

    Japanese Society for Aquaculture Science

References:  22

Cited by:  1

Codes

  • NII Article ID (NAID)
    10008020076
  • NII NACSIS-CAT ID (NCID)
    AN00124667
  • Text Lang
    JPN
  • Article Type
    Journal Article
  • ISSN
    03714217
  • NDL Article ID
    6115823
  • NDL Source Classification
    ZR26(科学技術--農林水産--水産)
  • NDL Call No.
    Z18-761
  • Data Source
    CJP  CJPref  NDL  J-STAGE  JASI 
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