ガザミ自然集団におけるミトコンドリアDNA-Dループ領域の制限酵素切断型多型

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  • Genetic Variability of Swimming Crab Based on PCR-RFLP Analysis of Mitochondrial DNA D-Loop Region.
  • ガザミ自然集団におけるミトコンドリアＤＮＡ‐Ｄループ領域の制限酵素切断型多型
  • ガザミ シゼン シュウダン ニ オケル ミトコンドリア DNA Dループ リョウイキ ノ セイゲン コウソ セツダンガタ タケイ

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Restriction fragment length polymorphism analysis was performed on Polymerase Chain Reaction amplified DNA fragments containing the D-loop region of swimming crab Portunus trituberculatus mitochondrial DNA (mtDNA) . A total of 234 individuals consisting of eight populations around Japan were surveyed with four restriction endonucleases (Dra I, Hinc II, Mbo I and PshB I), yielding a total of 28 composite haplotypes. Within population of haplotypic diversity (h) and nucleotide diversity (π) were ranging from 0.403 (Miyagi) to 0.903 (Okayama) and from 0.0020 (Miyagi) to 0.0101 (Shizuoka), respectively. The Miyagi population was highly distinct from all other populatons. The homogeneity test by a chi-square randomization analysis demonstrated significant differences between Miyagi population and all the other six populations examined, and between Kumamoto and other two populations, Sizuoka and Okayama. The dendrogram clustering based on nucleotide divergence between populations, showed no clustering group of georaphical cline. Significance of hapolotype diversity (h) and nucleotide diversity (π) values obtained of swimming crab populations in the present study were discussed together with the data in mud crabs, differences in the genetic diversity between the mtDNA whole region and D-loop region, and the effects of stocking of artificially propagated larvae to the genetic composition of local populations in the swimming crab.

収録刊行物

  • 水産増殖

    水産増殖 50 (1), 1-7, 2002

    日本水産増殖学会

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