ICSIの際の卵細胞膜穿破様式と卵の質および受精,初期発生との関連 Flexibility of Oolemma is an Important Factor for Oocyte Survival after ICSI

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Author(s)

    • 柳田 薫 YANAGIDA Kaoru
    • 福島県立医科大学部産科学婦人科学講座 Department of Obstetrics and Gynecology, Fukushima Medical University
    • 片寄 治男 KATAYOSE Haruo
    • 福島県立医科大学部産科学婦人科学講座 Department of Obstetrics and Gynecology, Fukushima Medical University
    • 菅沼 亮太 SUGANUMA Akira
    • 福島県立医科大学部産科学婦人科学講座 Department of Obstetrics and Gynecology, Fukushima Medical University
    • 佐藤 章 SATO Akira
    • 福島県立医科大学部産科学婦人科学講座 Department of Obstetrics and Gynecology, Fukushima Medical University
    • 近内 勝之 Sato Akira
    • 福島県立医科大学部産科学婦人科学講座 Department of Obstetrics and Gynecology, Fukushima Medical University

Abstract

卵細胞質内精子注入法(ICSI)の際の卵細胞膜穿破様式と卵の生存率,受精,分割率,ICSI前の卵の形態学的評価との関連性を検討した.ニードル先端の鋭利性による影響を除外するために,先端が平坦なニードルを使用できるピエゾマイクロマニピュレーターを用いてICSIを行った.ニードルによって卵細胞膜が穿破される様式を細胞膜の伸展性から4タイプに分類した.ICSIを行う成熟卵の約8%は卵細胞膜の伸展性が不良(type A)で,それらの50%はcytolysisを起こした.しかし,生存卵に対しての受精率,受精卵に対しての分割胚率に有意差がなかった.卵内に空胞が認められる不良卵と穿破様式との間にも関連性が認められなかった.

We examined the relationship between the breakage modes occurring during intracytoplasmic sperm injection (ICSI) and survival, fertilization and embryonic development rates after ICSI, and the morphologic quality of the oocytes before ICSI. The tip of each injection pipette was flattened to exclude the factor of tip shape. Oolemma breakage modes were grouped into four types according to oolemma flexibility. Approximately 8% of the mature oocytes treated with ICSI featured an inflexible oolemma, and 50% of such oocytes resulted in cytolysis, but no significant differences were observed in fertilization rates among the surviving oocytes, and no relationship could be traced between breakage modes and morphologic oocyte quality. The selection of oocytes with flexible oolemma is believed to be crucial in obtaining satisfactory results with ICSI, but it is not yet possible to distinguish oocytes that have inflexible oolemma from other oocytes before ICSI.

Journal

  • Journal of Mammalian Ova Research

    Journal of Mammalian Ova Research 18(3), 93-98, 2001-10-01

    JAPANESE SOCIETY OF OVA RESEARCH

References:  16

Codes

  • NII Article ID (NAID)
    10008024454
  • NII NACSIS-CAT ID (NCID)
    AN10548943
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    13417738
  • NDL Article ID
    5982024
  • NDL Source Classification
    ZR4(科学技術--生物学--動物)
  • NDL Call No.
    Z18-1673
  • Data Source
    CJP  NDL  J-STAGE  JASI 
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