Development of Automated Nuclear Transplantation System

• Tsukada Nobuhiro

  Dept. of Precision Engineering, the University of Tokyo

• Kudoh Ken-ichi

  Dept. of Precision Engineering, the University of Tokyo

• Budiman Michel

  Dept. of Precision Engineering, the University of Tokyo

• Yamamoto Akio

  Dept. of Precision Engineering, the University of Tokyo

• Higuchi Toshiro

  Dept. of Precision Engineering, the University of Tokyo

• Kobayashi Mamoru

  Nihon University, College of Bioresource Science

• Sato Kahei

  Nihon University, College of Bioresource Science

• Oishi Katsuaki

  Suruga Seiki Co., Ltd.

• Iida Katsuhiko

  Suruga Seiki Co., Ltd.

Bibliographic Information

Other Title

• 全自動核移植システムの開発

Abstract

In nuclear transplantation, especially in enucleation, rotating the oocyte to a certain position has been an important work. Therefore, we developed an oocyte rotation system by using electrostatic force, which is known to be excellent in handling minute objects. This system has become the basic technology for the automated nuclear transplantation system. Mouse oocyles were rotated at a speed of about 60 deg/s by electrostatic phenomena (i.e. electrorotation and dielectrophoresis), and the direction of rotation could be reversed. In order to confirm the electrostatic effect on the fertilization and developmental ability of oocytes, we performed IVF and nuclear transplantation with rotated oocytes. The result was that the fertilization and developmental ability of the rotated oocytes was the same as that of the control, proving that rotation by electrostatic force does not influence the fertilization and development ability of oocytes.

Journal

• Journal of Mammalian Ova Research

  Journal of Mammalian Ova Research 18 (3), 106-109, 2001

  JAPANESE SOCIETY OF OVA RESEARCH

Keywords

• Mammalian Oocyte
• Nuclear transplantation
• Micromanipulator
• Electrostatic force
• electrorotation
• dielectrophoresis

Details

• CRID: 1390282679315383936
• NII Article ID: 10008024500
• NII Article ID: 30002016784
• NII Book ID: AN10548943
• DOI: 10.1274/jmor.18.106
• ISSN: 13475878
• ISSN: 13417738
• NDL BIB ID: 5982044
• Web Site: https://agriknowledge.affrc.go.jp/RN/2030640754
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I5982044
• Text Lang: en
• Data Source:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed