牛卵母細胞体外成熟での透明帯遺伝子発現に及ぼすFSHおよびLHの影響 Effect of Follicle-stimulating Hormone and Luteinizing Hormone on Zona Pellucida Gene Expression during Bovine Oocyte Maturation In Vitro

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Author(s)

    • 渡辺 伸也 WATANABE Shinya
    • 農水省九州農業試験場|Present: Agriculture, Forestry and Fisheries Research Council Secretariat, Ministry of Agriculture, Forestry and Fisheries Kyushu National Agricultural Experimental Station

Abstract

牛卵母細胞体外成熟での透明帯遺伝子発現に及ぼすFSHおよびLHの影響をRNase protection assayで調べた。その結果,ホルモン処理区ではいずれも体外成熟5および15時間後の卵母細胞で透明帯遺伝子発現に影響が認められなかった。しかしながら,FSH区で卵核胞崩壊と減数分裂中期II誘起に有意な抑制が見られた.これらの結果から,FSHとLHは牛卵母細胞での透明帯遺伝子発現に関係がなく,この遺伝子発現はすくなくとも牛では卵母細胞の核成熟とは関連がないことが示唆された.

Follicle-stimulating hormone (FSH) and luteinizing hormone (LH) play important roles in bovine oocyte maturation. This study tested the effect of FSH and LH on bovine ZP gene expression during oocyte maturation in vitro with RNase protection assay. These gonadotropins had no effect on ZP gene expression in bovine oocytes cultured for 5 or 15 hours, but significant suppression of germinal vesicle breakdown (GVB) and metaphase II (MII) generation were observed with FSH. These data suggest that neither FSH nor LH has a significant effect on ZP gene expression in bovine oocytes. And the ZP gene expression could be independent of the mechanism of oocyte nuclear maturation in cattle.

Journal

  • Journal of Mammalian Ova Research

    Journal of Mammalian Ova Research 18(3), 116-118, 2001-10-01

    JAPANESE SOCIETY OF OVA RESEARCH

References:  13

Codes

  • NII Article ID (NAID)
    10008024540
  • NII NACSIS-CAT ID (NCID)
    AN10548943
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    13417738
  • NDL Article ID
    5982088
  • NDL Source Classification
    ZR4(科学技術--生物学--動物)
  • NDL Call No.
    Z18-1673
  • Data Source
    CJP  NDL  J-STAGE  JASI 
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