代謝プロファイルテストのためのウシ血液の採取・保存方法の検討  [in Japanese] Methods for Collection and Preservation of Cattle Blood for Metabolic Profile Test  [in Japanese]

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Author(s)

    • 岡田 啓司 OKADA K.
    • 岩手大学農学部 附属家畜病院 Veterinary Teaching Hospital, Faculty of Agriculture, Iwate University
    • 安田 準 YASUDA J.
    • 岩手大学農学部 附属家畜病院 Veterinary Teaching Hospital, Faculty of Agriculture, Iwate University

Abstract

代謝プロファイルテスト(MPT)における血液サンプルの採取・保存条件を、健康な黒毛和種成雌牛5頭を用いて検討した。血清分離剤を用いて速やかに分離した血清(血清)に比べて、ヘパリン加血漿は遊離脂肪酸(FFA)、βヒドロキシ酪酸(BHB)、総コレステロール(T-cho)、リン脂質(PL)、アスパラギン酸アミノトランスフェラーゼ(AST)、γグルタミルトランスペプチダーゼ(γ-GTP)が低値、βリポ蛋白(β-lipo)、総蛋白(Tp)、アルブミン(Alb)が高値を示した。全血を室温2時間放置後の血清は、尿素窒素(BUN)とγ-GTPが高値を示した。血糖(Glu)は血清とヘパリン加血漿に差はなかった。<BR>分離血清はGlu、BHB、T-cho、Tp、Alb、BUN、無機リンでは冷蔵・冷凍保存とも7日目まで安定していたが、FFA、PL、β-lipo、Ca、AST、γGPTは7日以前に変化が見られた。

Conditions for collecting and preserving blood samples for the metabolic profile test (MPT) were investigated by using five healthy adult castrated Japanese Black cattle. Values in heparinated plasma were compared with those in rapidly separated serum : free fatty acid (FFA), β hydroxy-butyric acid (BHB), total cholesterol (T-cho), phospholipids (PL), aspartate aminotransferase (AST) and γ glutamyl transpeptidase (γ-GTP) were lower in plasma, while β lipoprotein (β-lipo), total protein (Tp) and albumin (Alb) were higher in plasma. When whole blood was stored at room temperature for 2 hours, urea nitrogen (BUN) and γ-GTP were elevated in serum. Regarding glucose (Glu) level there was no difference between serum and heparinated plasma. When separated serum was refrigerated or frozen, Glu, BHB, T-cho, Tp, Alb, BUN and inorganic phosphor were stable for 7 days. FFA, PL, β-Lipo, Ca, AST and γ-GTP were relatively unstable.

Journal

  • Japanese Journal of Veterinary Clinics

    Japanese Journal of Veterinary Clinics 24(1), 13-18, 2001-07-20

    Japanese Society of Large Animal Clinics / Large Animal Clinic Research Association

References:  2

Codes

  • NII Article ID (NAID)
    10008024620
  • NII NACSIS-CAT ID (NCID)
    AA1163152X
  • Text Lang
    JPN
  • Article Type
    ART
  • ISSN
    09167579
  • NDL Article ID
    5848543
  • NDL Source Classification
    ZR22(科学技術--農林水産--畜産)
  • NDL Call No.
    Z18-2162
  • Data Source
    CJP  NDL  J-STAGE  JASI 
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