平板希釈法によるコンポスト化微生物計数を目的とした培養条件の検討 Culture Conditions for Determining Microbial Population Size Contributing to High Rate Composting of Dairy Manure by using the Dilution Agar-plate Method

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Abstract

コンポスト化反応に関わる細菌および放線菌の生菌数を把握するために, 平板希釈法における培養条件 (寒天培地, 培養温度, 培養時間) を検討した。生菌数計測用試料は乳牛ふんを断熱型リアクタで強制通気を行いながら作成したコンポストを用い, 種々の培養条件のもとで生菌数を測定し, それが最大となる条件を探索した。供試した寒天培地は細菌, 放線菌とも各4種類で, 設定温度は20から70℃まで5℃間隔で行った。その結果, 細菌数および放線菌数を測定する場合の最適な培養条件が見いだされ, 特に中温性細菌の培養温度については広く受け入れられている温度より高い約43℃が適温であった。

This paper describes the appropriate microbial culture conditions (agar medium, incubation temperature and incubation time), for estimating the microbial population size of bacteria and actinomycetes in dairy manure compost. A sample of dairy manure compost was prepared in a forced aeration adiabatic reactor. The microbial population sizes of the sample were then measured using the dilution agar-plate method with various culture conditions, to determine the best microbial culture condition. Four different agar mediums were tested at various incubation temperatures ranging from 20 to 70°C at 5°C intervals, to measure the population sizes of both bacteria and actinomycetes. We identified a suitable agar medium, incubation temperature and incubation time for determining the microbial population size of mesophilic and thermophilic bacteria and actinomycetes. Of particular note, we found that the optimum culture conditions for the mesophilic bacteria occurred at about 43°C, higher than temperatures that are widely accepted.

Journal

  • JOURNAL of the JAPANESE SOCIETY of AGRICULTURAL MACHINERY

    JOURNAL of the JAPANESE SOCIETY of AGRICULTURAL MACHINERY 63(6), 85-89, 2001-11-01

    The Japanese Society of Agricultural Machinery and Food Engineers

References:  9

Codes

  • NII Article ID (NAID)
    10008025314
  • NII NACSIS-CAT ID (NCID)
    AN00200470
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    02852543
  • NDL Article ID
    5967719
  • NDL Source Classification
    ZR7(科学技術--農林水産--農産)
  • NDL Call No.
    Z18-387
  • Data Source
    CJP  NDL  J-STAGE 
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