ＰＣＲ法によるＰｒｅｖｏｔｅｌｌａ属におけるβ‐ｌａｃｔａｍａｓｅ遺伝子の検索　　Ｐｒｅｖｏｔｅｌｌａ　ｎｉｇｒｅｓｃｅｎｓ　ＴＯ１６７由来ｃａｒｂａｐｅｎｅｍａｓｅ遺伝子の解析をもとに

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  • PCR detection of .BETA.-lactamase genes in Prevotella spp.: Based on analysis of Prevotella nigrescens TO167 carbapenemase gene.
  • Based on analysis of <I>Prevotella nigrescens</I> TO 167 carbapenemase gene
  • <I>Prevotella nigrescens</I> TO 167由来carbapenemase遺伝子の解析をもとに

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We studied carbapenemase gene cloning of anaerobic gram-negative rod Prevotella nigrescens TO 167 (MIC, imipenem resistant>32μg/mL) isolated from odontogenic infection and the distribution of β-lactamase genes in β-lactamase-producing Prevotella. The Prevotella nigrescens TO 167 carbapenemase gene was cloned in Escherichia coli 5 α transcojugants using PCR products of blaIMP, and the DNA fragment related to this enzyme considered to be extracted from the transconjugant was 1, 493 bp. DNA homologous for this carbapenemase gene and the β-lactamase gene from 13 gram-negative rods, including blaIMP, was 42.9-50.4%.β-lactamase genes were detected by PCR using Prevotella (81 strains) isolated from oral cavity. The 15 primers used in this study were designed from 14 β-lactamase genes including carbapenemase genes. Amplification by PCR with primers was observed in imipenem-susceptible strains of Prevotella: for Kp CAZ (class A), Ko ampCA (class C), and Ab OXA 21 (class D) from Prevotella intermedia; for Kp CAZ and Ab OXA 21 from Prevotella nigrescens; and for Ko ampCA from Prevotella loescheii. The β-Lactamase gene was not detected in imipenem-resistant strains. These results suggest that the Prevotella nigrescens TO 167 carbapenemase gene cloned differed from previous reported β-lactamase genes. We concluded that carbapenemase genes were different from known carbapenemase genes and that there is variety in Prevotella. And β-lactamase genes of Prevotella were similar to those of Klebsiella and Acinetobacter.

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