Purification of Serotype d- and e- specific Antigens from Actinobacillus actinomycetemcomitans and Seroclassification of Clinical Isolates from Periodontal Patients.

• Yamamoto Matsuo

  Department of Periodontology, Faculty of Dentistry, Tokyo Medical and Dental University

Bibliographic Information

Other Title

• Ａｃｔｉｎｏｂａｃｉｌｌｕｓ　ａｃｔｉｎｏｍｙｃｅｔｅｍｃｏｍｉｔａｎｓ　血清型ｄ，ｅの多糖抗原の精製および臨床分離株との関連について

Abstract

Oral Actinobacillus actinomycetemcomitans strains have been classified into five serotypes. The aim of this study was to determine the compositions of A. actinomycetemcomitans serotype d- and e- specific antigens. The serodistribution of clinical isolates from the patients with periodontitis were also investigated. Serotype-specific polysaccharide antigens of A. actinomycetemcomitans IDH 781 (serotype d) and OMZ 534 (serotype e) were extracted from whole cells by autoclaving. The extracts were purified by chromatography on DEAE-Sepharose CL-6B and Sephacryl S-300HR columns. The serotype d antigen was composed of rhamnose (17. 1%), mannose (45.5%), galactose (2.0%) and glucose (35.5%) . On the other hand, the serotype e antigen was composed of rhamnose (23.9%), mannose (29.1%), galactose (11.0%), glucose (13. 5%) and unidentified sugar (22.5%) . Immunodiffusion tests revealed that the purified polysaccharide antigen form a single precipitin line with the corresponding rabbit antiserum. A total of 157 A. actinomycetemcomitans clinical isolates from diseased sites of 39 patients with periodontitis were serotyped by using serotype-specific rabbit antisera against A. actinomycetemcomitans serotype a, b, c, d and e strains. In the immunodiffusion assay, the autoclaved extracts of 42, 12, 34, 8 and 41 A. actinomycetemcomitans clinical isolates reacted with serotype a, b, c, d and e antisera, respectively. These findings indicate that the extraction of serotype antigens by autoclaving is useful and definite for the serotyping of A. actinomycetemcomitans clinical isolates.

Journal

• THE JOURNAL OF THE STOMATOLOGICAL SOCIETY,JAPAN

  THE JOURNAL OF THE STOMATOLOGICAL SOCIETY,JAPAN 63 (1), 174-187, 1996

  The Stomatological society, Japan

Keywords

• General Medicine

Details

• CRID: 1390001206341572224
• NII Article ID: 130004282775
• NII Article ID: 10008140294
• NII Book ID: AN00302674
• DOI: 10.5357/koubyou.63.174
• ISSN: 18845185
• ISSN: 03009149
• PubMed: 8725365
• Web Site: https://search.jamas.or.jp/link/ui/1996186032
• Text Lang: ja
• Data Source:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed