培養三叉神経節ニューロンにおけるグルタミン酸ならびにGABA受容体の機能特性

  • 飯田 穣
    東京医科歯科大学歯学部歯科矯正学第一講座 東京医科歯科大学歯学部口腔生理学講座

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  • Functional Properties of Receptors for Glutamate and GABA in Cultured Trigeminal Ganglion Neurons.

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Properties of receptors for glutamate, consisting of kainate, α-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), and N-methyl -D-aspartate (NMDA), and γ-aminobutyric acid (GABA) in the rat trigeminal ganglion (TG) neurons were studied by means of the wholecell patchclamp technique.<BR>1. All TG neurons (diameter 20-40μm) responded to GABA, but none of them responded to AMPA, NMDA, and glycine. TG neurons responding to kainate were smaller than 25μm and desensitized by prolonged agonist exposure.<BR>2. On the basis of the doseresponse curves, EC50 and Hill coefficient were found to be 13.7μM and 1.2 for kainate, while those for GABA were 28.6μM and 2.0, respectively.<BR>3. Most kainate receptors in the TG neurons showed linear current-voltage (I-V) relations, though some showed inward rectification. The “Instantaneous” I-V relationship for GABA was linear, where as the “steady-state” I-V relationship produced by ramp changes in potential showed outward rectification in some neurons.<BR>4. The number of TG neurons responding to kainate increased until the 8th postnatal day, while all the recorded neurons responded to GABA in postnatal days.<BR>The results indicated that there are kainate and GABAa receptors in the TG neurons and suggested that they are composed of various subunits.

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