Differential Display法を用いたOsteogenic Protein-1(OP-1)の骨形成作用に伴う細胞内シグナル伝達に関与する遺伝子の解析  [in Japanese] Analysis of Genes Implicated in Osteogenic Signaling by Osteogenic Protein-1 (OP-1) using Differential Display Method  [in Japanese]

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Author(s)

    • 小山 治彦 KOYAMA Haruhiko
    • 東京医科歯科大学歯学部口腔外科学第二講座 The Second Department of Oral and Maxillofacial Surgery,Faculty of Dentistry, Tokyo Medical and Dental University

Abstract

Bone morphogenetic proteins (BMPs), members of a transforming growth factor-β (TGF-β) superfamily, are growth and differentiation factors which induce ectopic bone formation<I>in vivo.</I>Although many studies on osteoinductive properties of BMPs have been conducted, little is known about the downstream components in the signal transduction machinery, beyond the mechanism of BMP receptor activation.<BR>In this study, the osteogenic effects by osteogenic protein-1 (OP-1, BMP-7) on osteoblastic cell line MC3T3-E1 and murine stromal cell line ST2 were investigated, especially focusing on differentially expressed genes induced by OP-1 using the differential display method.<BR>The major findings were as follows:<BR>1) Alkaline phosphatase specific activities of both MC3T3-E1 and ST2 increased in a dosedependent manner by OP-1 stimulation.<BR>2) Northern analysis showed a significant increase of osteocalcin mRNA after 7 days of OP-1 treatment.<BR>3) 77 genes, which were differentially expressed in MC 3 T 3-E 1 and ST 2 cells, were detected on differential display fingerprints after 16-hour treatment of OP-1.<BR>4) Some of these clones showed high levels of identical to known genes.<BR>5) One clone called ST3v, down-regulated in ST2 cells by OP-1 stimulation, was confirmed with quantitative RT-PCR.

Journal

  • THE JOURNAL OF THE STOMATOLOGICAL SOCIETY,JAPAN

    THE JOURNAL OF THE STOMATOLOGICAL SOCIETY,JAPAN 64(2), 205-222, 1997-06-30

    The Stomatological society, Japan

References:  46

Codes

  • NII Article ID (NAID)
    10008141929
  • NII NACSIS-CAT ID (NCID)
    AN00302674
  • Text Lang
    JPN
  • Article Type
    ART
  • ISSN
    03009149
  • Data Source
    CJP  J-STAGE 
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