Ｄｉｆｆｅｒｅｎｔｉａｌ　Ｄｉｓｐｌａｙ法を用いたＯｓｔｅｏｇｅｎｉｃ　Ｐｒｏｔｅｉｎ‐１（ＯＰ‐１）の骨形成作用に伴う細胞内シグナル伝達に関与する遺伝子の解折

• 小山 治彦

  東京医科歯科大学歯学部口腔外科学第二講座

書誌事項

タイトル別名

• Analysis of Genes Implicated in Osteogenic Signaling by Osteogenic Protein-1 (OP-1) using Differential Display Method.

抄録

Bone morphogenetic proteins (BMPs), members of a transforming growth factor-β (TGF-β) superfamily, are growth and differentiation factors which induce ectopic bone formationin vivo.Although many studies on osteoinductive properties of BMPs have been conducted, little is known about the downstream components in the signal transduction machinery, beyond the mechanism of BMP receptor activation.<BR>In this study, the osteogenic effects by osteogenic protein-1 (OP-1, BMP-7) on osteoblastic cell line MC3T3-E1 and murine stromal cell line ST2 were investigated, especially focusing on differentially expressed genes induced by OP-1 using the differential display method.<BR>The major findings were as follows:<BR>1) Alkaline phosphatase specific activities of both MC3T3-E1 and ST2 increased in a dosedependent manner by OP-1 stimulation.<BR>2) Northern analysis showed a significant increase of osteocalcin mRNA after 7 days of OP-1 treatment.<BR>3) 77 genes, which were differentially expressed in MC 3 T 3-E 1 and ST 2 cells, were detected on differential display fingerprints after 16-hour treatment of OP-1.<BR>4) Some of these clones showed high levels of identical to known genes.<BR>5) One clone called ST3v, down-regulated in ST2 cells by OP-1 stimulation, was confirmed with quantitative RT-PCR.

収録刊行物

• 口腔病学会雑誌

  口腔病学会雑誌 64 (2), 205-222, 1997

  口腔病学会

キーワード

• General Medicine

詳細情報

• CRID: 1390001206342146176
• NII論文ID: 10008141929
• NII書誌ID: AN00302674
• DOI: 10.5357/koubyou.64.205
• ISSN: 18845185
• ISSN: 03009149
• PubMed: 9232957
• Web Site: https://search.jamas.or.jp/link/ui/1998008476
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可