根管から分離された<i>Prevotella intermedia</i>と<i>Prevotella nigrescens</i>について

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  • <i>Prevotella intermedia</i> and <i>Prevotella nigrescens</i> isolated from root canal

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<p>Abstract : Anaerobic, Gram-negative, black-pigmented rods (BPRs) have been thought to be one of putative periodontic and endodontic pathogenes.</p><p> Recently, one of BPRs, Prevotella intemedia, was reclassified into P. intermedia and P. nigrescens. Many investigators have determined the occurence of these bacteria in periodontal diseases using new classification, however, there is only few reports to estimate the occurence of them in apical periodontitis.</p><p> The purpose of this study was to investigate the occurrence of P. intermedia and P. nigrescens in apical periodontitis including the teeth which have been done partial pulp treatments and the teeth without any pulp treatments. Subjects in this study were 8 teeth diagnosed as apical periodontitis. After removing their filling of the teeth, endodontic entry was done using rubber dam, and infected apical dentin was taken by H-file. The tip of file was cut by sterilized cutter, put into 1 ml of GAM broth and performed serial 10-fold dilutions with GAM broth. One hundred μl aliquot of each dilution was put on Brucella HK agar supplemented with 5% sheep blood, hemine and menadion and cultivated in Anaero Box under an atmosphere of 80% N2, 10% CO2 and 5% H2 for 7 days. All BPRs were isolated and classified into Genus Porphyromonas and Prevotella with performing Rapid ID 32 A system, fluorescence under long-wave of ultra violet (360 nm), trypsin-like enzyme activity, α-glucocidase activity. Furthermore, Genus Prevotella identified into P. intermedia and P. nigrescens using the difference of migration pattern of glutamate dehydrogenase and malate dehydrogenase.</p><p> In the teeth which have been done partial pulp treatments, facultative anaerobic bacteria were predominant while anaerobic bacteria were predominant in the teeth without any endodontic treatments. BPRs predominated among the isolates from the teeth which showed big radiolucent images at the apical lesions.</p><p> P. nigrescens was seldom isolated although P. intemedia was the most predominant species among the identified BPRs.</p>

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