根管から分離されたPrevotella intermediaとPrevotella nigrescensについて  [in Japanese] Prevotella intermedia and Prevotella nigrescens isolated from root canal  [in Japanese]

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Author(s)

    • 長田 玲子 OSADA Reiko
    • 鶴見大学歯学部第二歯科保存学教室 Department of Periodontics and Endodontics,Tsurumi University School of Dental Medicine
    • 石川 博之 ISHIKAWA Hiroyuki
    • 鶴見大学歯学部第二歯科保存学教室 Department of Periodontics and Endodontics,Tsurumi University School of Dental Medicine
    • 山口 博康 YAMAGUCHI Hiroyasu
    • 鶴見大学歯学部第二歯科保存学教室 Department of Periodontics and Endodontics,Tsurumi University School of Dental Medicine
    • 中村 治郎 NAKAMURA Jiro
    • 鶴見大学歯学部第二歯科保存学教室 Department of Periodontics and Endodontics,Tsurumi University School of Dental Medicine
    • 前田 伸子 MAEDA Nobuko
    • 鶴見大学歯学部口腔細菌学教室 Department of Oral Bacteriology, Tsurumi University School of Dental Medicine

Abstract

<p><b>Abstract : </b>Anaerobic, Gram-negative, black-pigmented rods (BPRs) have been thought to be one of putative periodontic and endodontic pathogenes.</p><p> Recently, one of BPRs, <i>Prevotella intemedia</i>, was reclassified into <i>P. intermedia</i> and <i>P. nigrescens</i>. Many investigators have determined the occurence of these bacteria in periodontal diseases using new classification, however, there is only few reports to estimate the occurence of them in apical periodontitis.</p><p> The purpose of this study was to investigate the occurrence of <i>P. intermedia</i> and <i>P. nigrescens</i> in apical periodontitis including the teeth which have been done partial pulp treatments and the teeth without any pulp treatments. Subjects in this study were 8 teeth diagnosed as apical periodontitis. After removing their filling of the teeth, endodontic entry was done using rubber dam, and infected apical dentin was taken by H-file. The tip of file was cut by sterilized cutter, put into 1 m<i>l</i> of GAM broth and performed serial 10-fold dilutions with GAM broth. One hundred μ<i>l</i> aliquot of each dilution was put on Brucella HK agar supplemented with 5% sheep blood, hemine and menadion and cultivated in Anaero Box under an atmosphere of 80% N<sub>2</sub>, 10% CO<sub>2</sub> and 5% H<sub>2</sub> for 7 days. All BPRs were isolated and classified into Genus <i>Porphyromonas</i> and <i>Prevotella</i> with performing Rapid ID 32 A system, fluorescence under long-wave of ultra violet (360 nm), trypsin-like enzyme activity, α-glucocidase activity. Furthermore, Genus <i>Prevotella</i> identified into <i>P. intermedia</i> and <i>P. nigrescens</i> using the difference of migration pattern of glutamate dehydrogenase and malate dehydrogenase.</p><p> In the teeth which have been done partial pulp treatments, facultative anaerobic bacteria were predominant while anaerobic bacteria were predominant in the teeth without any endodontic treatments. BPRs predominated among the isolates from the teeth which showed big radiolucent images at the apical lesions.</p><p> <i>P. nigrescens</i> was seldom isolated although <i>P. intemedia</i> was the most predominant species among the identified BPRs.</p>

Journal

  • The Journal of Japan Endodontic Association

    The Journal of Japan Endodontic Association 19(1), 24-30, 1998-06-25

    Japan Endodontic Association

References:  22

Codes

  • NII Article ID (NAID)
    10008154174
  • NII NACSIS-CAT ID (NCID)
    AN10443184
  • Text Lang
    JPN
  • Article Type
    ART
  • ISSN
    13406248
  • Data Source
    CJP  J-STAGE 
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