デンチャープラークおよび歯石様沈着物の電子顕微鏡的観察  [in Japanese] Electron Microscopic Study of Denture Plaque and Denture Calculus  [in Japanese]

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Author(s)

    • 石川 千恵子 ISHIKAWA Chieko
    • 鶴見大学歯学部歯科補綴学第一講座 The First Department of Prosthetic Dentistry, Tsurumi University School of Dental Medicine

Abstract

The formation processes of the denture plaque and calculus were studied by means of transmission electron microscopy and EPMA. The dentures worn experimentally for 6 hours and 3 days and the dentures obtained from patients were used.<BR>For the electron microscopy, the pieces cut from the dentures were embedded in Epok 812 after fixation, and the ultrathin sections were examined without staining. For EPMA, the cut pieces were immediately frozen in liquid nitrogen, and the freeze-dried sections were prepared and examined.<BR>Amorphous electron dense materials appeared in the spaces between the loosely bound microorganisms on the denture surface at 3 days, although the denture plaque was not observed at 6 hours. The relatively high X-ray peak of calcium was detected from the plaque. The lamellated substance appeared on the surface of the denture from the patients, and the substance was heavily mineralized. The initial mineral crystals appeared along the plasma membrane of microorganisms, the intermicrobiol matrix, and the lamellated substance.<BR>These results suggested that the denture plaque facilitates the calcium accumulation and the formation of the denture calculus is initiated by the intermicrobiol matrix, the cell membrane, and/or the lamellated substance.

Journal

  • Nihon Hotetsu Shika Gakkai Zasshi

    Nihon Hotetsu Shika Gakkai Zasshi 41(6), 985-997, 1997-12-10

    Japan Prosthodontic Society

References:  53

Cited by:  5

Codes

  • NII Article ID (NAID)
    10008168192
  • NII NACSIS-CAT ID (NCID)
    AN00197911
  • Text Lang
    JPN
  • Article Type
    Journal Article
  • ISSN
    03895386
  • Data Source
    CJP  CJPref  J-STAGE 
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