ラット心筋 in vivo マイクロダイアリシス法  [in Japanese] In vivo microdialysis technique on rat myocardium  [in Japanese]

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Author(s)

Abstract

マイクロダイアリシス法は近年めざしましい進歩をとげ, 精力的に行われているが, 心臓は他の臓器と異なり拍動するため心筋への応用は困難とされてきた. 著者の開発したマイクロダイアリシス法はプローブと心臓の動きを連動させ, プローブと組織との摩擦を除去し, 心臓に対する負荷を和らげるよう工夫したものであり, 長時間の生体物質の測定が可能である. 本稿ではin vivoマイクロダイアリシス法により心筋中に発生するラジカルの測定法, アデノシン産生によるエクト5’-ヌクレオチダーゼ活性の評価法について概説する. これらの方法によって得られたin vivo評価法は心臓疾患のメカニズムなどの解明に応用が可能で, 有益な情報をもたらすものと思われる.

The microdialysis procedure is a technique that has been established for some years. The heart, however, has several difficulties in the application of this technique. The heart is the beating in vivo, in contrast to other organs. I developed the flexibly mounted microdialysis technique, which involves the synchronized movement of the tip of the probe with the beating heart to reduce tissue injury. With this technique, it is feasible to make stable and long-term measurements of interstitial biological substances. By employing the flexibly mounted microdialysis technique, I will demonstrate that the monitoring of free radical generation and the level of adenosine measured during AMP perfusion that gives an index of the activity of ecto-5’-nucleotidase in the tissue. In the future, this technique will have versatile applications for useful studies to elucidate the actual mechanism in the pathogenesis of heart disorders.

Journal

  • Folia Pharmacologica Japonica

    Folia Pharmacologica Japonica 118(6), 379-382, 2001-12-01

    The Japanese Pharmacological Society

References:  14

Codes

  • NII Article ID (NAID)
    10008181456
  • NII NACSIS-CAT ID (NCID)
    AN00198335
  • Text Lang
    JPN
  • Article Type
    NOT
  • ISSN
    00155691
  • NDL Article ID
    5995050
  • NDL Source Classification
    ZS51(科学技術--薬学)
  • NDL Call No.
    Z19-247
  • Data Source
    CJP  NDL  J-STAGE 
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