スフィンゴシン1-リン酸定量法の確立とその応用  [in Japanese] Establishment of the method for the measurement of sphingosine-1-phosphate in biological samples and its application for S1P research  [in Japanese]

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Author(s)

    • 岡島 史和 OKAJIMA Fumikazu
    • 群馬大学生体調節研究所調節因子部門シグナル伝達分野 Laboratory of Signal Transudation, Institute for Molecular and Cellular Regulation, Gunma University

Abstract

S1Pは細胞増殖, 分化, 接着, 運動, アポトーシスのような様々な細胞機能に関与していることが知られてきた. このS1Pは当初, 細胞内ターゲットを介して作用すると考えられていたが, Gタンパク質連関受容体を介し, 細胞外シグナル伝達分子として機能する場合があることも判ってきた. S1Pの生理機能, 病態生理における役割を知るためには, 作用解析のみならず, S1Pの動態制御を知ることも重要である. 我々は最近, S1Pの定量法として, S1P受容体の一つEdg-1を強発現した細胞上での標識S1Pと検体中S1Pの競合反応を利用したラジオレセプターアッセイ法を開発した. 本稿では従来法との比較とその問題点, また, 我々がこの測定法を用いどのような研究をおこなっているか, 今後の展望などについて述べた.

Sphingosine 1-phosphate (S1P), one of the spingolipid metabolites, has been shown to participate in a variety of cellular responses including proliferation, differentiation, adhesion, motility, and apoptosis. These cellular responses elicited by S1P were first thought to be mediated through an intracellular target(s), but extracellular mechanisms through G-protein-coupled S1P receptors have also been suggested. In addition to the studies examining the functions of the lipid on the cells and tissues, the measurement of the lipid concentration is also important for understanding the physiological and pathophysiological roles of the lipid. We have recently developed a novel quantitative method for measurement of S1P, which was based on the competition of S1P in the samples with the labeled S1P on the S1P receptor Edg-1. Here, we compared our method with previously published ones in several points including specificity and simplicity. We further presented our recent results obtained by using this novel quantitative method and finally mentioned the prospects of the S1O measurement in lipid research, especially in relation to several disorders.

Journal

  • Folia Pharmacologica Japonica

    Folia Pharmacologica Japonica 118(6), 383-388, 2001-12-01

    The Japanese Pharmacological Society

References:  20

Cited by:  1

Codes

  • NII Article ID (NAID)
    10008181471
  • NII NACSIS-CAT ID (NCID)
    AN00198335
  • Text Lang
    JPN
  • Article Type
    Journal Article
  • ISSN
    00155691
  • NDL Article ID
    5995075
  • NDL Source Classification
    ZS51(科学技術--薬学)
  • NDL Call No.
    Z19-247
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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